Coleman D L, Culver K E, Ryan J L
Cell Immunol. 1985 Apr 1;91(2):520-7. doi: 10.1016/0008-8749(85)90250-3.
Macrophage phagocytic activity is regulated by a variety of products derived from activated lymphocytes. It has been reported that nonactivated splenic B and T lymphocytes enhance macrophage glucose metabolism. In addition, the enhancement of macrophage glucose metabolism was further increased by direct effects of bacterial lipopolysaccharide (LPS) on B, but not T, lymphocytes. In the present study, the effect of purified murine splenic B and T lymphocytes on Fc-dependent phagocytosis by thioglycollate-elicited peritoneal macrophages in the presence or absence of LPS has been investigated. Fc-dependent phagocytosis was assayed by measuring the ingestion of 51Cr-tagged sheep erythrocytes. After 3 or 4 days in culture, nonadherent spleen cells (NASC) and B and T lymphocytes from C3H/HeN (LPS-responder) mice produced 92 +/- 27%, 83 +/- 13%, and 147 +/- 33% increases in C3H/HeJ (LPS-hyporesponder) macrophage phagocytic activity, respectively. A similar effect was observed in Balb/c mice. Cell-free supernatant from NASC and B lymphocytes precultured for 2 or 4 days produced a 74 +/- 20% and 157 +/- 42% increase in phagocytosis respectively. At concentrations which have been previously shown to markedly enhance the ability of splenic B lymphocytes to stimulate macrophage glucose metabolism, Escherichia coli K235 LPS (10 micrograms/ml) did not alter the stimulatory effects of any of the splenic lymphocyte populations on macrophage Fc-dependent phagocytosis. These data suggest that B lymphocytes produce a soluble factor(s) which stimulates macrophage phagocytosis. In addition, LPS has different effects on the regulation of macrophage phagocytic activity and metabolism by B lymphocytes.
巨噬细胞的吞噬活性受多种活化淋巴细胞衍生产物的调节。据报道，未活化的脾脏B淋巴细胞和T淋巴细胞可增强巨噬细胞的葡萄糖代谢。此外，细菌脂多糖（LPS）对B淋巴细胞而非T淋巴细胞的直接作用可进一步增强巨噬细胞的葡萄糖代谢。在本研究中，研究了纯化的小鼠脾脏B淋巴细胞和T淋巴细胞在有或无LPS存在的情况下对巯基乙酸诱导的腹腔巨噬细胞Fc依赖性吞噬作用的影响。通过测量51Cr标记的绵羊红细胞的摄取来测定Fc依赖性吞噬作用。培养3或4天后，来自C3H/HeN（LPS反应者）小鼠的非贴壁脾细胞（NASC）以及B淋巴细胞和T淋巴细胞分别使C3H/HeJ（LPS低反应者）巨噬细胞的吞噬活性提高了92±27%、83±13%和147±33%。在Balb/c小鼠中也观察到了类似的效果。预先培养2或4天的NASC和B淋巴细胞的无细胞上清液分别使吞噬作用提高了74±20%和157±42%。在先前已证明能显著增强脾脏B淋巴细胞刺激巨噬细胞葡萄糖代谢能力的浓度下，大肠杆菌K235 LPS（10微克/毫升）并未改变任何脾脏淋巴细胞群体对巨噬细胞Fc依赖性吞噬作用的刺激效果。这些数据表明B淋巴细胞产生一种可刺激巨噬细胞吞噬作用的可溶性因子。此外，LPS对B淋巴细胞调节巨噬细胞吞噬活性和代谢具有不同的作用。
