Department of Biology, Faculty of Science, Aydin Adnan Menderes University, 09100, Aydin, Türkiye.
Department of Biology, Faculty of Science, Aydin Adnan Menderes University, 09100, Aydin, Türkiye.
J Invertebr Pathol. 2024 Jul;205:108126. doi: 10.1016/j.jip.2024.108126. Epub 2024 May 10.
Aedes-transmitted arboviral infections such as Dengue, Yellow Fever, Zika and Chikungunya are increasing public health problems. Xenorhabdus and Photorhabdus bacteria are promising sources of effective compounds with important biological activities. This study investigated the effects of cell-free supernatants of X. szentirmaii, X. cabanillasii and P. kayaii against Ae. aegypti eggs and larvae and identified the bioactive larvicidal compound in X. szentirmaii using The EasyPACId method. Among the three tested bacterial species, X. cabanillasii exhibited the highest (96%) egg hatching inhibition and larvicidal activity (100% mortality), whereas P. kayaii was the least effective species in our study. EasyPACId method revealed that bioactive larvicidal compound in the bacterial supernatant was fabclavine. Fabclavines obtained from promoter exchange mutants of different bacterial species such as X. cabanillasii, X. budapestensis, X. indica, X. szentirmaii, X. hominckii and X. stockiae were effective against mosquito larvae. Results show that these bacterial metabolites have potential to be used in integrated pest management (IPM) programmes of mosquitoes.
埃及伊蚊传播的虫媒病毒感染，如登革热、黄热病、寨卡病毒和基孔肯雅热，正成为日益严重的公共卫生问题。华丽色杆菌属和发光杆菌属的细菌是具有重要生物活性的有效化合物的有前途的来源。本研究调查了 X. szentirmaii、X. cabanillasii 和 P. kayaii 的无细胞上清液对埃及伊蚊卵和幼虫的影响，并使用 The EasyPACId 方法鉴定了 X. szentirmaii 中的生物活性杀幼虫化合物。在测试的三种细菌中，X. cabanillasii 表现出最高的（96%）卵孵化抑制和杀幼虫活性（100%死亡率），而 P. kayaii 在我们的研究中效果最差。EasyPACId 方法表明，细菌上清液中的生物活性杀幼虫化合物是法布菌素。法布菌素从不同细菌物种的启动子交换突变体中获得，如 X. cabanillasii、X. budapestensis、X. indica、X. szentirmaii、X. hominckii 和 X. stockiae，对蚊子幼虫有效。结果表明，这些细菌代谢物有可能用于蚊子的综合虫害管理（IPM）计划。
