Schuessler H, Hartmann H
Int J Radiat Biol Relat Stud Phys Chem Med. 1985 May;47(5):509-21. doi: 10.1080/09553008514550741.
Radiation-induced degradation of double-stranded DNA from calf thymus in aqueous solution with sodium phosphate was studied by conventional gel chromatography and by high-performance liquid-gel permeation chromatography. Comparison of the data after radiolysis of aqueous solutions of DNA under anaerobic and aerobic conditions indicates that double-strand breakage is not enhanced by oxygen. An increase of ionic strength impedes the break-down of the DNA molecules, so that loss of DNA can only be observed at doses above 100 Gy. Only reactions of OH-radicals contribute to the fragmentation of DNA, while the presence of hydrated electrons, H.-or formate radicals does not lead to a loss of highly polymerized DNA up to doses of 1500 Gy. High-performance liquid-chromatography proved to be an excellent method of studying the degradation of macromolecules as a function of dose.
通过传统凝胶色谱法和高效液相凝胶渗透色谱法研究了磷酸钠水溶液中辐射诱导的小牛胸腺双链DNA降解。对厌氧和好氧条件下DNA水溶液辐解后的数据进行比较表明，氧不会增强双链断裂。离子强度的增加会阻碍DNA分子的分解，因此只有在剂量高于100 Gy时才能观察到DNA的损失。只有OH自由基的反应会导致DNA断裂，而在剂量高达1500 Gy时，水合电子、H·或甲酸根自由基的存在不会导致高度聚合的DNA损失。高效液相色谱法被证明是研究大分子降解与剂量关系的一种出色方法。
