College of Environment and Ecology, Chongqing University, Chongqing, 400045, PR China.
College of Environment and Ecology, Chongqing University, Chongqing, 400045, PR China; The Key Laboratory of Eco-Environment in Three Gorges Reservoir Region, Chongqing University, Chongqing, 400045, PR China.
Chemosphere. 2024 Jul;359:142271. doi: 10.1016/j.chemosphere.2024.142271. Epub 2024 May 9.
Acinetobacter sp. AL-6 combining with biochar was adapted in activated sludge (AS & co-system) to decontaminate Mn, Fe and NH-N, and treat activated sludge (AS) for its activity and settling performance improvement. Specifically, the co-system promoted the growth of bacteria in the activated sludge, thus increasing its ability to nitrify and adsorb Mn and Fe, resulting in the removal of high concentrations of NH-N, Mn, Fe and COD in the reactor by 100%, 100%, 100%, and 96.8%, respectively. And the pH of wastewater was increased from 4 to 8.5 by co-system also facilitated the precipitation of Mn and Fe. The MLVSS/MLSS ratio increased from 0.64 to 0.95 and SVI decreased from 92.54 to 1.54 after the addition of co-system, which indicated that biochar helped to improve the activity and settling performance of activated sludge and prevented it from being damaged by the compound Mn and Fe. In addition, biochar promoted the increase of the tyrosine-like protein substance and humic acid-like organic matter in the sludge EPS, thus enhanced the ability of sludge to adsorb Mn and Fe. Concretely, compared with AS group, the proteins content and polysaccharides content of the AS & co-system group were increased by 13.14 times and 6.30 times respectively. Further, microbial diversity analysis showed that more resistant bacteria and dominant bacteria Acinetobacter sp. AL-6 in sludge enhanced the nitrification and adsorption of manganese and iron under the promotion of biochar. Pre-eminently, the more effective AS & co-system were applied to the removal of actual electrolytic manganese slag leachate taken from the contaminated site, and the removal of NH-N, Mn, Fe and COD remained high at 100%, 100%, 71.82% and 94.72%, respectively, revealing advanced value for high engineering applications of AS & co-system.
不动杆菌 sp.AL-6 与生物炭结合后，被应用于活性污泥（AS 和共系统）中，以去除 Mn、Fe 和 NH-N，并改善活性污泥（AS）的活性和沉降性能。具体而言，该共系统促进了活性污泥中细菌的生长，从而提高了其硝化和吸附 Mn 和 Fe 的能力，使反应器中 NH-N、Mn、Fe 和 COD 的去除率分别达到 100%、100%、100%和 96.8%。此外，共系统还将废水的 pH 值从 4 提高到 8.5，有利于 Mn 和 Fe 的沉淀。添加共系统后，MLVSS/MLSS 比值从 0.64 增加到 0.95，SVI 从 92.54 降低到 1.54，表明生物炭有助于提高活性污泥的活性和沉降性能，并防止其受到复合 Mn 和 Fe 的破坏。此外，生物炭促进了污泥 EPS 中酪氨酸样蛋白物质和腐殖酸类有机物的增加，从而增强了污泥吸附 Mn 和 Fe 的能力。具体来说，与 AS 组相比，AS 和共系统组的蛋白质含量和多糖含量分别增加了 13.14 倍和 6.30 倍。此外，微生物多样性分析表明，更多的抗性细菌和优势菌不动杆菌 sp.AL-6 在生物炭的促进下，增强了对锰和铁的硝化和吸附作用。突出的是，更有效的 AS 和共系统被应用于去除来自污染场地的实际电解锰渣浸出液，NH-N、Mn、Fe 和 COD 的去除率仍保持在 100%、100%、71.82%和 94.72%，显示出 AS 和共系统在高工程应用中的先进价值。
