Institute for Forest Resources and Environment of Guizhou, Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou Province, College of Forestry, Guizhou University, Guiyang 550025, Guizhou, China.
Institute for Forest Resources and Environment of Guizhou, Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou Province, College of Forestry, Guizhou University, Guiyang 550025, Guizhou, China.
Int J Biol Macromol. 2024 Jun;270(Pt 1):132235. doi: 10.1016/j.ijbiomac.2024.132235. Epub 2024 May 9.
Gleditsia sinensis Lam. is a multifaceted plant with medicinal, edible, chemical, timber, and ornamental applications. However, the effect of rootstocks on scions after grafting is still unclear. This study examined the mRNA and miRNA transcriptome among homografts, heterografts, and seedlings. GO enrichment analysis between seedlings and homograft/heterograft combinations revealed that biosynthesis, degradation, and transport were enriched. The KEGG enrichment results showed that plant hormone signal transduction and the plant MAPK signaling pathway were enriched in both seedlings and heterograft combinations. Through weighted correlation network analysis (WGCNA), the hub genes related to the content of plant hormones were obtained. Taking G. sinensis as the scion, there were 4594, 2887, 3429, and 5959 mRNAs that were specifically expressed in the grafted plants of G. sinensis/G. fera, G. sinensis/G. delavayi, G. sinensis/G. microphylla, and G. sinensis/G. japonica, respectively. The specifically expressed mRNA genes may participate in such processes and pathways as the rhythmic process, circadian rhythm, gibberellic-acid-mediated signaling pathway, and peptide-based amino acid modification. Additionally, 3, 16, 2, and 15 specifically expressed miRNAs were identified. This study examines the impact of grafting on gene expression in Gleditsia plants and establishes a foundation for the development of new resources and rootstock breeding.
皂荚是一种多用途植物，具有药用、食用、化工、木材和观赏价值。然而，接穗在砧木上嫁接后的效果仍不清楚。本研究考察了同源嫁接、异源嫁接和实生苗之间的 mRNA 和 miRNA 转录组。在实生苗和同源/异源嫁接组合之间的 GO 富集分析显示，生物合成、降解和运输是富集的。KEGG 富集结果表明，植物激素信号转导和植物 MAPK 信号通路在实生苗和异源嫁接组合中均有富集。通过加权相关网络分析（WGCNA），获得了与植物激素含量相关的枢纽基因。以皂荚为接穗，在皂荚/金雀儿、皂荚/甘蒙锦鸡儿、皂荚/小果皂荚和皂荚/日本皂荚的嫁接植株中，分别有 4594、2887、3429 和 5959 个 mRNAs 特异性表达。这些特异性表达的 mRNA 基因可能参与节律过程、昼夜节律、赤霉素介导的信号通路和基于肽的氨基酸修饰等过程和途径。此外，还鉴定了 3、16、2 和 15 个特异性表达的 miRNA。本研究考察了嫁接对皂荚植物基因表达的影响，为新资源的开发和砧木的选育奠定了基础。
