Rothstein T L
J Immunol. 1985 Jul;135(1):106-10.
The ability of cytochalasin to influence the stimulation of murine B lymphocytes through surface immunoglobulin was assessed during short term cultures. Modest doses of anti-immunoglobulin alone did not stimulate proliferation of mouse spleen cells at 2 days. Cytochalasin B alone also had no effect. However, anti-immunoglobulin in combination with cytochalasin B stimulated substantial proliferation as judged by [3H]thymidine incorporation. Cytochalasins A, E, and D, and dihydrocytochalasin B were all effective in promoting B cell proliferation. Spleen cells from xid-defective (CBA/N X DBA/2)F1 male mice failed to proliferate in response to anti-immunoglobulin plus cytochalasin, suggesting that this treatment affects the same subset of B cells as anti-immunoglobulin plus B cell growth factor. Moreover, proliferation that was stimulated by anti-immunoglobulin plus cytochalasin B was not affected by T cell depletion. Cytochalasin may circumvent the need for, or replace, a second signal for proliferation.
在短期培养过程中评估了细胞松弛素通过表面免疫球蛋白影响小鼠B淋巴细胞刺激的能力。单独使用适量的抗免疫球蛋白在2天时不会刺激小鼠脾细胞增殖。单独使用细胞松弛素B也没有效果。然而，抗免疫球蛋白与细胞松弛素B联合使用时，通过[3H]胸苷掺入判断可刺激大量增殖。细胞松弛素A、E和D以及二氢细胞松弛素B在促进B细胞增殖方面均有效。来自xid缺陷型（CBA/N×DBA/2）F1雄性小鼠的脾细胞在抗免疫球蛋白加细胞松弛素的作用下未能增殖，这表明这种处理影响的B细胞亚群与抗免疫球蛋白加B细胞生长因子相同。此外，抗免疫球蛋白加细胞松弛素B刺激的增殖不受T细胞耗竭的影响。细胞松弛素可能绕过对增殖第二信号的需求或替代该信号。
