SANKEN, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, Japan.
Graduate School of Frontier Biosciences, Osaka University, Yamadaoka 1-3, Suita, Osaka, Japan.
Biosci Biotechnol Biochem. 2024 Jul 22;88(8):908-917. doi: 10.1093/bbb/zbae060.
We analyzed the effects of olfactory receptors (ORs) on transient receptor potential vanilloid 1 (TRPV1) activation using HEK293T cells co-expressing TRPV1 and OR51E1. We demonstrate here that the effect of OR51E1 on TRPV1 activation varies depending on the two TRPV1 ligands: capsaicin and eugenol. Notably, both of these ligands are vanilloid analogs. OR51E1 enhanced the response of TRPV1 to capsaicin but diminished that to eugenol. OR51E2 also showed similar effects. Based on the susceptibility to the OR's modulatory effects, various TRPV1 ligands could be classified into capsaicin and eugenol types. Activation of OR51E1 enhanced cAMP production. In addition, forskolin exhibited almost identical effects as ORs on TRPV1 responses to both types of ligands. These results suggest that OR51E1-induced cAMP elevation leads to a modification of TRPV1, presumably phosphorylation of TRPV1, which amplifies the susceptibility of TRPV1 to the two types of ligands differently.
我们使用共表达 TRPV1 和 OR51E1 的 HEK293T 细胞分析了嗅觉受体 (ORs) 对瞬时受体电位香草酸 1 (TRPV1) 激活的影响。我们在此证明，OR51E1 对 TRPV1 激活的影响取决于两种 TRPV1 配体：辣椒素和丁香酚。值得注意的是，这两种配体都是香草酸类似物。OR51E1 增强了 TRPV1 对辣椒素的反应，但减弱了对丁香酚的反应。OR51E2 也表现出类似的效果。基于对 OR 调节作用的敏感性，各种 TRPV1 配体可分为辣椒素和丁香酚型。OR51E1 的激活增强了 cAMP 的产生。此外， forskolin 对 TRPV1 对这两种配体的反应表现出与 OR 几乎相同的作用。这些结果表明，OR51E1 诱导的 cAMP 升高导致 TRPV1 的修饰，推测 TRPV1 的磷酸化，这使得 TRPV1 对这两种类型的配体的敏感性不同。
