Song Jingjing, Bai Hongying, Chen Si, Xing Yuanyuan, Lou Jiyu
Department of Neurology, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou, 450014, China.
Heliyon. 2024 Apr 26;10(9):e30422. doi: 10.1016/j.heliyon.2024.e30422. eCollection 2024 May 15.
Galectins-8 (Gal-8), the tandem repeat sequences of the galectin family, can influence the pathophysiologic processes in neurological disorders. However, its effect on intracerebral hemorrhage and related mechanisms remains nebulous. Using collagenase VII-S-induced ICH in the left striatum of mice, we investigated the effects of Gal-8 on cellular and molecular immune inflammatory responses in hemorrhagic brain and evaluated the severity of short- and long-term brain injury. Our results showed that activated microglia in the periphery of hematoma in mice with intracerebral hemorrhage expressed Gal-8, while Gal-8 could regulate the expression of cytokines, such as HMGB-1 ( = 0.0032), TNF-α ( = 0.0158), and IL-10 ( = 0.0379). Inhibition of the glucose-binding activity of Gal-8 by thiogalactoside (TDG) significantly reduced the volume of cerebral hematoma ( = 0.0241) and hydrocephalus ( = 0.0112) during the acute phase of cerebral hemorrhage and improved the long-term prognosis. TDG can reduce acute-phase brain tissue injury and improve the prognosis by inhibiting the activation of immune-inflammatory cells in the periphery of hematoma and reducing the release of pro-inflammatory factors.
半乳糖凝集素-8（Gal-8）是半乳糖凝集素家族的串联重复序列，可影响神经疾病的病理生理过程。然而，其对脑出血的影响及相关机制仍不明确。我们利用胶原酶VII-S诱导小鼠左侧纹状体脑出血，研究了Gal-8对出血性脑内细胞和分子免疫炎症反应的影响，并评估了短期和长期脑损伤的严重程度。我们的结果显示，脑出血小鼠血肿周围活化的小胶质细胞表达Gal-8，而Gal-8可调节细胞因子的表达，如高迁移率族蛋白B1（P = 0.0032）、肿瘤坏死因子-α（P = 0.0158）和白细胞介素-10（P = 0.0379）。硫代半乳糖苷（TDG）抑制Gal-8的葡萄糖结合活性可显著减少脑出血急性期的脑血肿体积（P = 0.0241）和脑积水（P = 0.0112），并改善长期预后。TDG可通过抑制血肿周围免疫炎症细胞的活化和减少促炎因子的释放来减轻急性期脑组织损伤并改善预后。
