通过连续尾静脉注射筛选小鼠黑色素瘤细胞中与转移相关的基因。
Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection.
作者信息
Hao Qinggang, Dong Rui, Bai Weiyu, Chang Dong, Yao Xinyi, Zhang Yingru, Xu Huangying, Li Huiyan, Kui Xiang, Wang Feng, Wang Yan, Wang Chengqin, Lei Yujie, Chen Yan, Shen Junling, Sang Lei, Bai Yan, Sun Jianwei
机构信息
Center for Life Sciences, Yunnan Key Laboratory of Cell Metabolism and Diseases, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming 650500, China.
Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, Yan'an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
出版信息
Biophys Rep. 2024 Feb 29;10(1):15-21. doi: 10.52601/bpr.2023.230043.
Tumor metastasis, responsible for approximately 90% of cancer-associated mortality, remains poorly understood. Here in this study, we employed a melanoma lung metastasis model to screen for metastasis-related genes. By sequential tail vein injection of mouse melanoma B16F10 cells and the subsequently derived cells from lung metastasis into BALB/c mice, we successfully obtained highly metastatic B16F15 cells after five rounds of screening. RNA-sequencing analysis of B16F15 and B16F10 cells revealed a number of differentially expressed genes, some of these genes have previously been associated with tumor metastasis while others are novel discoveries. The identification of these metastasis-related genes not only improves our understanding of the metastasis mechanisms, but also provides potential diagnostic biomarkers and therapeutic targets for metastatic melanoma.
肿瘤转移导致了约90%的癌症相关死亡，但其机制仍知之甚少。在本研究中，我们采用黑色素瘤肺转移模型来筛选与转移相关的基因。通过将小鼠黑色素瘤B16F10细胞经尾静脉连续注射，并将随后从肺转移灶获得的细胞再注射到BALB/c小鼠体内，经过五轮筛选，我们成功获得了高转移性的B16F15细胞。对B16F15和B16F10细胞进行RNA测序分析，发现了许多差异表达基因，其中一些基因先前已被证实与肿瘤转移相关，而其他一些则是新发现。这些与转移相关基因的鉴定不仅增进了我们对转移机制的理解，也为转移性黑色素瘤提供了潜在的诊断生物标志物和治疗靶点。
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