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鞘磷脂磷酸二酯酶酸性样3B对卵巢癌细胞增殖、迁移和侵袭的作用

Contribution of sphingomyelin phosphodiesterase acid-like 3B to the proliferation, migration, and invasion of ovarian cancer cells.

作者信息

Song Baozhi, Jiang Yu, Lin Ying, Liu Jiahua, Jiang Yatao

机构信息

Department of Gynecology, Shengli Clinical Medical College of Fujian Medical University & Fujian Provincial Hospital, Fuzhou, China.

Department of Pathology, Shengli Clinical Medical College of Fujian Medical University & Fujian Provincial Hospital, Fuzhou, China.

出版信息

Transl Cancer Res. 2024 Apr 30;13(4):1954-1968. doi: 10.21037/tcr-24-309. Epub 2024 Apr 25.

DOI:10.21037/tcr-24-309
PMID:38737677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11082662/
Abstract

BACKGROUND

Cancer has the highest mortality rate among gynecological cancers and poses a serious threat to women's lives. However, the treatment options for ovarian cancer are still limited, and exploring effective targeted biomarkers is particularly important for predicting and treating ovarian cancer. Therefore, it is necessary to explore the molecular mechanisms of the occurrence and development of ovarian cancer.

METHODS

This investigation encompassed the analysis of gene expression profiles, measurement of transcription levels of potential target genes in peripheral blood samples from ovarian cancer patients and characterization of the ovarian cancer-related secretory protein sphingomyelin phosphodiesterase acid-like 3B (SMPDL3B). Through bioinformatics analysis, potential target genes were identified, and their association with overall survival (OS) and progression-free survival (PFS) in ovarian cancer patients was assessed utilizing relevant databases. Subsequently, differences in target gene expression in ovarian cancer tissue samples were validated through protein blotting and quantitative real-time PCR (qRT-qPCR). Cell proliferation assays using the cell count kit-8 (CCK-8) method, as well as transwell chamber assay and pre coated matrix gel chamber assay were employed to elucidate the role of SMPDL3B in ovarian cancer cell migration and invasion.

RESULTS

This study revealed a substantial upregulation of in the serum of ovarian cancer patients, correlating with an unfavorable prognosis. High expression was linked not only to increased proliferation of ovarian cancer cells, but also enhanced migration and invasion. Remarkably, the knockdown the human alkaline ceramidase 2 () gene in cancer cells with heightened expression significantly inhibited cell proliferation, migration, and invasion induced by activation (P<0.05), highlighting the functional interplay between and in ovarian cancer.

CONCLUSIONS

In summary, this study proposes as a prognostic marker for ovarian cancer, with implications for potential therapeutic intervention targeting the ACER2-SMPDL3B axis.

摘要

背景

癌症在妇科癌症中死亡率最高，对女性生命构成严重威胁。然而，卵巢癌的治疗选择仍然有限，探索有效的靶向生物标志物对于预测和治疗卵巢癌尤为重要。因此，有必要探究卵巢癌发生发展的分子机制。

方法

本研究包括分析基因表达谱、测量卵巢癌患者外周血样本中潜在靶基因的转录水平以及对卵巢癌相关分泌蛋白酸性鞘磷脂磷酸二酯酶样3B（SMPDL3B）进行表征。通过生物信息学分析确定潜在靶基因，并利用相关数据库评估其与卵巢癌患者总生存期（OS）和无进展生存期（PFS）的关联。随后，通过蛋白质印迹和定量实时PCR（qRT-qPCR）验证卵巢癌组织样本中靶基因表达的差异。采用细胞计数试剂盒-8（CCK-8）法进行细胞增殖测定，以及Transwell小室测定和预包被基质胶小室测定，以阐明SMPDL

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/c6997984a39c/tcr-13-04-1954-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/3c549a7723ef/tcr-13-04-1954-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/1b02b2028475/tcr-13-04-1954-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/83239a47461f/tcr-13-04-1954-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/85e72d1e1c0d/tcr-13-04-1954-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/446a152061d3/tcr-13-04-1954-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/c6997984a39c/tcr-13-04-1954-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/3c549a7723ef/tcr-13-04-1954-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/1b02b2028475/tcr-13-04-1954-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/83239a47461f/tcr-13-04-1954-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/85e72d1e1c0d/tcr-13-04-1954-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/446a152061d3/tcr-13-04-1954-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc7/11082662/c6997984a39c/tcr-13-04-1954-f6.jpg

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