Richens E R, Seward M E, Groves R W
Acta Diabetol Lat. 1985 Jan-Mar;22(1):55-62. doi: 10.1007/BF02591093.
A method is described for the measurement of T-cell cytotoxicity to purified preparations of insulin, their high molecular weight fractions and C-peptide derivatives. The technique assesses cellular cytotoxicity to membrane associated insulin in an autologous system. Peripheral blood lymphocytes from 19 type 1 diabetic patients were stimulated in vitro for 6 days with the pharmaceutical preparation(s) used therapeutically. At the end of this period, they were set up in a 4-h cytotoxicity assay against 51Cr labelled PHA blast transformed lymphocytes in the presence of therapeutic insulin, monocomponent insulin, B-component or C-peptide. The results presented here demonstrate cytotoxicity to insulin B-component in diabetic patients who have received pork insulin for 2-6 years. The possible reasons for this finding are discussed.
本文描述了一种用于测量T细胞对纯化胰岛素制剂、其高分子量组分和C肽衍生物的细胞毒性的方法。该技术在自体系统中评估细胞对膜相关胰岛素的细胞毒性。来自19名1型糖尿病患者的外周血淋巴细胞在体外与治疗用药物制剂一起刺激6天。在此期间结束时，在治疗性胰岛素、单组分胰岛素、B组分或C肽存在的情况下，将它们用于针对51Cr标记的PHA blast转化淋巴细胞的4小时细胞毒性测定。此处给出的结果证明了接受猪肉胰岛素治疗2至6年的糖尿病患者对胰岛素B组分具有细胞毒性。讨论了这一发现的可能原因。
