Laboratorio de Investigaciones en Osteopatías y Metabolismo Mineral (LIOMM), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Department of Physiological Sciences, University of Florida, Gainesville, Florida, USA.
Exp Clin Endocrinol Diabetes. 2024 Oct;132(10):547-556. doi: 10.1055/a-2324-8661. Epub 2024 May 13.
We previously showed that a 3-week oral metformin (MET) treatment enhances the osteogenic potential of bone marrow stromal cells (BMSCs) and improves several bone histomorphometric parameters in Wistar rats with metabolic syndrome (MetS). However, the skeletal effects of extended periods of MET need to be completely elucidated. Hence, in this study, the impact of a prolonged (3-month) MET treatment was investigated on bone architecture, histomorphometric and biomechanics variables, and osteogenic potential of BMSCs in Wistar rats with or without MetS.
Young male Wistar rats (n=36) were randomized into four groups (n=9) that received either 20% fructose (), MET (), F plus MET treatments (), or drinking water alone (). Rats were euthanized, blood was collected, and bones were dissected and processed for peripheral quantitative computed tomography (pQCT) analysis, static and dynamic histomorphometry, and bone biomechanics. In addition, BMSCs were isolated to determine their osteogenic potential.
MET affected trabecular and cortical bone, altering bone architecture and biomechanics. Furthermore, MET increased the pro-resorptive profile of BMSCs. In addition, fructose-induced MetS practically did not affect the the structural or mechanical variables of the skeleton.
A 3-month treatment with MET (with or without MetS) affects bone architecture and biomechanical variables in Wistar rats.
我们之前的研究表明，3 周的口服二甲双胍（MET）治疗可增强骨髓基质细胞（BMSCs）的成骨潜能，并改善代谢综合征（MetS）大鼠的几种骨组织形态计量学参数。然而，需要完全阐明 MET 延长治疗的骨骼效应。因此，在这项研究中，研究了延长（3 个月）MET 治疗对 Wistar 大鼠骨结构、组织形态计量和生物力学变量以及 BMSCs 成骨潜能的影响，无论是否存在 MetS。
将雄性 Wistar 大鼠（n=36）随机分为四组（n=9），分别接受 20%果糖（）、MET（）、F 和 MET 联合治疗（）或单独饮用水（）。大鼠安乐死后，采集血液，分离骨骼进行外周定量计算机断层扫描（pQCT）分析、静态和动态组织形态计量学以及骨生物力学分析。此外，分离 BMSCs 以确定其成骨潜能。
MET 影响骨小梁和皮质骨，改变骨结构和生物力学。此外，MET 增加了 BMSCs 的促再吸收特征。此外，果糖诱导的 MetS 实际上对骨骼的结构或机械变量没有影响。
3 个月的 MET 治疗（无论是否存在 MetS）都会影响 Wistar 大鼠的骨结构和生物力学变量。
