Kan M, Kato M, Yamane I
In Vitro Cell Dev Biol. 1985 Mar;21(3 Pt 1):181-8. doi: 10.1007/BF02621356.
Human umbilical vein endothelial cells (HUV-EC) grew rapidly in vitro in medium supplemented with epidermal growth factor, fetal bovine serum (FBS) and human diploid fibroblast-conditioned medium. The effect of FBS could be replaced partially by bovine serum albumin, cholesterol, and vitamin E, and completely by further addition of serum dialysate or refeeding every other day. Among these components, fibroblast-conditioned medium is essential for HUV-EC growth. The HUV-EC were cultured serially for over 50 population doublings in the 10% FBS containing fibroblast-conditioned medium and for over 40 population doublings in the serum-free medium. Mitogenic factor(s) present in the medium conditioned by fibroblasts may be related to endothelial cell growth factor and play an important role in angiogenesis and regeneration of vascular endothelium in vitro.
人脐静脉内皮细胞（HUV-EC）在添加表皮生长因子、胎牛血清（FBS）和人二倍体成纤维细胞条件培养基的培养基中可在体外快速生长。FBS的作用可部分被牛血清白蛋白、胆固醇和维生素E替代，通过进一步添加血清透析液或每隔一天换液可完全替代。在这些成分中，成纤维细胞条件培养基对HUV-EC生长至关重要。HUV-EC在含10%FBS的成纤维细胞条件培养基中连续传代培养超过50次群体倍增，在无血清培养基中连续传代培养超过40次群体倍增。成纤维细胞条件培养基中存在的促有丝分裂因子可能与内皮细胞生长因子有关，在体外血管生成和血管内皮再生中起重要作用。
