GBF-Gesellschaft für Biotechnologische Forschung mbH., Mascheroder Weg 1, D-3000, Braunschweig, FRG.
Cytotechnology. 1989 Aug;2(3):171-9. doi: 10.1007/BF00133242.
Various polypeptide growth factors, culture substrates, basal media, sera and further supplements were assayed for improvement of growth of human vascular endothelial cells from umbilical cord veins. The resulting optimized medium consisted of gelatinized culture substrates, a mixture (1:1) of Iscove's MDM and Ham's F12 basal media supplemented with 20% newborn calf serum, 500 ng/ml crude fibroblast growth factor, 20 ng/ml epidermal growth factor, 5 μg/ml transferrin, 5 μg/ml insulin and 10 μg/ml heparin. The medium allowed long term cultivation of HUVEC up to 45 generations with maximal cell densities of about 10(5) cells per cm(2) and a minimal doubling time of about 14 hours at low cell densities.
各种多肽生长因子、培养底物、基础培养基、血清和进一步的补充剂被检测用于提高人脐静脉血管内皮细胞的生长。优化后的培养基由胶化培养底物、Iscove 的 MDM 和 Ham 的 F12 基础培养基（1:1）混合物组成，补充有 20%新生牛血清、500ng/ml 粗成纤维细胞生长因子、20ng/ml 表皮生长因子、5μg/ml 转铁蛋白、5μg/ml 胰岛素和 10μg/ml 肝素。该培养基允许 HUVEC 长期培养至 45 代，最高细胞密度约为每平方厘米 10(5)个细胞，在低细胞密度下最小倍增时间约为 14 小时。
