Programme in Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, Singapore.
Programme in Cardiovascular & Metabolic Disorders, Duke-National University of Singapore Medical School, Singapore, Singapore.
Methods Mol Biol. 2024;2808:105-120. doi: 10.1007/978-1-0716-3870-5_8.
Measles is a highly infectious disease that continues to spread mainly in developing countries, often resulting in child mortality. Despite the existence of effective vaccines, no specific antivirals are available as targeted therapy to combat measles virus (MeV). The implementation of genome-wide siRNA screens can provide a powerful platform to discover host factors that mediate MeV infection and replication, which could be essential to develop novel therapeutic strategies against this disease. Here, we describe a human genome-wide siRNA screen for MeV.
麻疹是一种高度传染性疾病，主要在发展中国家传播，常导致儿童死亡。尽管存在有效的疫苗，但作为针对麻疹病毒（MeV）的靶向治疗方法，尚无可用的特定抗病毒药物。全基因组 siRNA 筛选的实施可以为发现介导 MeV 感染和复制的宿主因素提供一个强大的平台，这对于开发针对这种疾病的新型治疗策略可能至关重要。在这里，我们描述了一种用于 MeV 的人类全基因组 siRNA 筛选。
