CIRI, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, Université de Lyon, Université Claude Bernard Lyon 1, École Normale Supérieure de Lyon, Lyon, France.
PST-A, University of Tours, Tours, France.
Methods Mol Biol. 2024;2808:167-175. doi: 10.1007/978-1-0716-3870-5_13.
Measles virus is one of the most contagious airborne human viruses which keeps causing outbreaks in numerous countries over the world despite the existence of an efficient vaccine. Fusion inhibitory lipopeptides were shown to inhibit viral entry into target cells, and their adequate administration into the respiratory tract may provide a novel preventive approach against airborne infections. Aerosol delivery presents the best administration route to deliver such preventive compounds to the upper and lower respiratory tract. This approach offers a conceptually new strategy to protect the population at risk against infection by respiratory viruses, including measles. It is a noninvasive needle-free approach, which may be used when antiviral protection is required, without any medical assistance. In this chapter, we describe the nebulization approach of lipopeptide compounds in nonhuman primates and the subsequent measles virus challenge.
麻疹病毒是最具传染性的空气传播人类病毒之一，尽管存在有效的疫苗，但它仍在世界上许多国家不断引发疫情。融合抑制性脂肽已被证明可抑制病毒进入靶细胞，将其充分施用于呼吸道可能为预防空气传播感染提供一种新方法。气溶胶输送是将此类预防化合物递送至上呼吸道和下呼吸道的最佳给药途径。这种方法为保护处于危险中的人群免受包括麻疹在内的呼吸道病毒感染提供了一种全新的策略。这是一种非侵入性、无针的方法，在需要抗病毒保护时无需任何医疗协助即可使用。在本章中，我们描述了脂肽化合物在非人灵长类动物中的雾化方法以及随后的麻疹病毒挑战。
