State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian, Liaoning 116034, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian, Liaoning 116034, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, Liaoning 116034, China; Dalian Key Laboratory for Precision Nutrition, Dalian Polytechnic University, Dalian, Liaoning 116034, China.
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian, Liaoning 116034, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian, Liaoning 116034, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, Liaoning 116034, China; Dalian Key Laboratory for Precision Nutrition, Dalian Polytechnic University, Dalian, Liaoning 116034, China.
Colloids Surf B Biointerfaces. 2024 Jul;239:113960. doi: 10.1016/j.colsurfb.2024.113960. Epub 2024 May 10.
The controlled release of antioxidant substances at the intestinal oxidative damage site is crucial for alleviating intestine-related diseases. Herein, the novel ROS-responsive carrier was synthesized through simple amidation reaction between carboxymethyl chitosan (CMC) and methionine (Met), a natural organic compound containing ROS-responsive linkages (thioether). Initially, astaxanthin (AXT) nanoparticles (AXT@CMT) with excellent stability and drug loading capacity (39.68 ± 0.23 μg/mL) were prepared by optimizing various reaction conditions. In the simulated high-concentration ROS environment of the intestine, CMT achieved a transition from hydrophobic groups (thioether) into hydrophilic groups (sulfone), which was conducive to the controlled release of AXT. In vitro cell experiments revealed that AXT@CMT could effectively alleviate the oxidative damage in intestinal epithelioid cell line No. 6 (IEC-6 cell) caused by HO. This study achieved a straightforward preparation of ROS-responsive nanocarrier through food ingredients, offering a theoretical foundation for the controlled release of AXT at the intestinal oxidative damage site.
在肠道氧化损伤部位控制抗氧化物质的释放对于缓解肠道相关疾病至关重要。在此，通过羧甲基壳聚糖（CMC）和含 ROS 响应性键（硫醚）的天然有机化合物蛋氨酸（Met）之间的简单酰胺化反应合成了新型的 ROS 响应性载体。首先，通过优化各种反应条件，制备了具有优异稳定性和药物载量（39.68±0.23μg/mL）的姜黄素纳米颗粒（AXT@CMT）。在肠道中高浓度 ROS 的模拟环境中，CMT 从疏水性基团（硫醚）转变为亲水性基团（砜），有利于 AXT 的控制释放。体外细胞实验表明，AXT@CMT 能够有效缓解 HO 引起的肠道上皮细胞系 No.6（IEC-6 细胞）的氧化损伤。本研究通过食品成分直接制备了 ROS 响应性纳米载体，为 AXT 在肠道氧化损伤部位的控制释放提供了理论基础。
Colloids Surf B Biointerfaces. 2024-7
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