Hussain Iftak, Rial Clara, Boza Juan, Tompkins Sheridan, Branen Josh, Giordano Julio, Erickson David
Sibley School of Mechanical and Aerospace Engineering Cornell University, Ithaca, NY, 14850, USA.
Department of Animal Science, Cornell University, Ithaca, NY, 14853, USA.
Anal Bioanal Chem. 2024 Jul;416(18):4101-4109. doi: 10.1007/s00216-024-05333-6. Epub 2024 May 14.
Reproductive management significantly impacts dairy farm productivity, necessitating accurate timely pregnancy detection in cattle. This paper presents a novel handheld and portable fluorescence imaging system designed for quantitative assessment of pregnancy-specific biomarkers, addressing the limitations of current detection methods. The objective was to develop a cost-effective, at-farm solution for detecting pregnancy-specific protein B (PSPB) in bovine plasma samples. The system integrates an imaging module and a custom software application, enabling image capture, data processing, and PSPB concentration determination. Calibration utilizing known PSPB concentrations achieved a 0.6 ng/mL limit of detection. Validation encompassed a comparison with a standard ELISA method using 100 bovine plasma samples; minimal bias and good agreement were observed within the linear range of the calibration curve for both methods. The system offers portability, user-friendliness, and potential for multiplex detection, promising real-time, at-farm reproductive management. This study demonstrates the successful development and validation of a portable fluorescence imaging system, offering an efficient and accurate approach to detecting pregnancy-specific biomarkers in cattle. Its implications extend to improving dairy farm productivity by enabling timely and reliable reproductive management practices.
繁殖管理对奶牛场的生产力有重大影响，因此需要对牛进行准确及时的妊娠检测。本文介绍了一种新型手持式便携式荧光成像系统，该系统旨在对妊娠特异性生物标志物进行定量评估，以解决当前检测方法的局限性。其目标是开发一种经济高效的农场级解决方案，用于检测牛血浆样本中的妊娠特异性蛋白B（PSPB）。该系统集成了一个成像模块和一个定制软件应用程序，能够进行图像采集、数据处理以及PSPB浓度测定。利用已知的PSPB浓度进行校准，检测限达到0.6 ng/mL。验证过程包括使用100份牛血浆样本与标准ELISA方法进行比较；在两种方法校准曲线的线性范围内，观察到最小偏差和良好的一致性。该系统具有便携性、用户友好性以及多重检测的潜力，有望实现农场级实时繁殖管理。本研究证明了便携式荧光成像系统的成功开发和验证，为检测牛妊娠特异性生物标志物提供了一种高效准确的方法。其意义在于通过实现及时可靠的繁殖管理实践来提高奶牛场的生产力。
