Gebreslassie Sara, Jida Mulissa, Puente Mariana Laura, Covacevich Fernanda, Belay Zerihun
Department of Applied Biology, Adama Science and Technology University, Adama, Ethiopia.
Bio and Emerging Technology Institute, Ras Biru Street, Near TemenJa Yaj, Addis Ababa, Ethiopia.
Int J Microbiol. 2024 May 7;2024:9226715. doi: 10.1155/2024/9226715. eCollection 2024.
Arbuscular mycorrhizal fungi (AMF) and some rhizobacteria are known as plant growth-promoting microorganism (PGPM) as they play significant roles in improving soil fertility structure, plant nutrition, growth, and health. However, little is known about the PGPM potential of AMF and rhizobacteria native to the Rift Valley and highland regions of Ethiopia. Hence, this study aimed to investigate the PGPM effect of single and co-inoculation of AMF and the ALCR46 strain, on tomato ( L.), onion ( L.), and squash ( L.) plants. The experimental setup was a randomized complete block design with three replications of the following treatments: (i) inoculation with a consortium of AMF, (ii) co-inoculation with a consortium of AMF and the , (iii) inoculation with , (iv) co-inoculation with and , (v) inoculation with , (vi) plants without inoculation (negative control), and (vii) plants treated with chemical fertilizer (positive control). Plants were maintained in a greenhouse for 60 days, and after harvest, plant growth parameters, percentage of AMF root colonization, and spore number were analyzed. The result shows that the growth of crops significantly increased by co-inoculation with the consortium of AMF and . AMF spore density and root colonization rate were also increased in co-inoculated plants. Highest root colonization, spore number, and mycorrhizal dependency were observed in . Our results suggest that there is a synergistic effect between the AMF and ALCR46, and between AMF inoculants. However, the application of present findings under field conditions is required to be confirmed by further studies.
丛枝菌根真菌（AMF）和一些根际细菌被称为植物生长促进微生物（PGPM），因为它们在改善土壤肥力结构、植物营养、生长和健康方面发挥着重要作用。然而，对于埃塞俄比亚裂谷和高地地区原生的AMF和根际细菌的PGPM潜力知之甚少。因此，本研究旨在调查AMF与ALCR46菌株单独接种和共同接种对番茄（L.）、洋葱（L.）和南瓜（L.）植株的PGPM效应。实验设置为随机完全区组设计，对以下处理进行三次重复：（i）接种AMF菌剂联合体，（ii）接种AMF菌剂联合体与ALCR46菌株，（iii）接种ALCR46菌株，（iv）接种ALCR46菌株与AMF菌剂，（v）接种AMF菌剂，（vi）未接种植株（阴性对照），以及（vii）用化肥处理的植株（阳性对照）。将植株在温室中培养60天，收获后分析植株生长参数、AMF根定殖百分比和孢子数。结果表明，接种AMF菌剂联合体与ALCR46菌株显著提高了作物的生长。共同接种的植株中AMF孢子密度和根定殖率也有所增加。在接种ALCR46菌株与AMF菌剂的处理中观察到最高的根定殖率、孢子数和菌根依赖性。我们的结果表明，AMF与ALCR46菌株之间以及AMF接种剂之间存在协同效应。然而，目前的研究结果在田间条件下的应用还需要进一步研究来证实。
