Passwell J, Shor R, Keren G, el-On J
Clin Exp Immunol. 1985 Jun;60(3):565-71.
Human monocytes were infected in vitro with Leishmania tropica major (L. major) promastigotes which transformed to intracellular amastigotes. A spontaneous increase in lymphocyte proliferation occurred in mononuclear cell cultures where the monocytes had been infected with L. major organisms. In addition, an apparent additive effect of lymphocyte proliferation was seen in cultures infected with L. major following phytohaemagglutinin stimulation. This effect was apparent after 3 days in culture and the amount of increase in response was dependent on the number of monocytes in the culture. The effect was also dependent on the number of parasites ingested by the monocytes. The presence of monocytes was essential for this effect, as no enhancement was observed with supernatants from infected cells. This enhanced effect of lymphocyte proliferation was observed predominantly in the B lymphocyte subpopulation. These findings may be of relevance in the immunopathogenesis of Leishmania infections.
人单核细胞在体外被热带利什曼原虫（硕大利什曼原虫）前鞭毛体感染，这些前鞭毛体转化为细胞内无鞭毛体。在单核细胞被硕大利什曼原虫感染的单核细胞培养物中，淋巴细胞增殖出现自发增加。此外，在经植物血凝素刺激后被硕大利什曼原虫感染的培养物中，可见淋巴细胞增殖的明显相加效应。这种效应在培养3天后明显，反应增加的量取决于培养物中单核细胞的数量。该效应还取决于单核细胞摄取的寄生虫数量。单核细胞的存在对这种效应至关重要，因为感染细胞的上清液未观察到增强作用。淋巴细胞增殖的这种增强效应主要在B淋巴细胞亚群中观察到。这些发现可能与利什曼原虫感染的免疫发病机制有关。
