Singh A K, Makker S P
Clin Exp Immunol. 1985 Jun;60(3):579-85.
A monospecific antiserum to gp600, a recently isolated and characterized antigen of Heymann nephritis (HN), was used in radioimmunoassay to measure the specific activity of the antigen in various rat tissues. Lung contained the highest amount followed by pancreas, stomach, small intestine, kidney, large intestine, seminal vesicle, epididymis, muscle, heart, brain, spleen, liver, testis and serum. Immunoprecipitation of the antigen with anti-gp600 and analyses of the precipitates on SDS-PAGE revealed that the majority of the tissues contained a cross-reactive 90 kD polypeptide antigen. Four of these tissues also contained a 66kD polypeptide antigen. Seminal vesicles, brain, kidney and liver contained a high mol. wt (greater than 330kD) antigen. Kidney possessed a unique set of antigens, namely 330kD, 110kD and triplet of 80kD besides the common 90kD and greater than 330kD antigens. These results show that antigens cross-reactive with HN gp600 antigen are present in other tissues but the biochemical nature of the kidney antigen is unique in comparison to antigen present in other tissues.
一种针对gp600（最近分离并鉴定的海曼肾炎（HN）抗原）的单特异性抗血清被用于放射免疫测定，以测量该抗原在各种大鼠组织中的比活性。肺中含量最高，其次是胰腺、胃、小肠、肾脏、大肠、精囊、附睾、肌肉、心脏、大脑、脾脏、肝脏、睾丸和血清。用抗gp600对抗原进行免疫沉淀，并在SDS-PAGE上对沉淀物进行分析，结果显示大多数组织含有一种交叉反应性的90kD多肽抗原。其中四个组织还含有一种66kD多肽抗原。精囊、大脑、肾脏和肝脏含有一种高分子量（大于330kD）抗原。除了常见的90kD和大于330kD抗原外，肾脏还拥有一组独特的抗原，即330kD、110kD和80kD的三联体。这些结果表明，与HN gp600抗原交叉反应的抗原存在于其他组织中，但与其他组织中存在的抗原相比，肾脏抗原的生化性质是独特的。
