Colussi D, Marfil F, Sioufi A
J Chromatogr. 1985 Apr 12;339(1):157-62. doi: 10.1016/s0378-4347(00)84637-x.
A method for the simultaneous determination of pirprofen and its metabolite, the pyrrole derivative, in human plasma is described. The two compounds and the butyric acid analogue of the pyrrole derivative used as internal standard are extracted from plasma with chloroform, then back-extracted into an alkaline buffer. After addition of acid, the aqueous phase is assayed by high-performance liquid chromatography using a fixed-wavelength ultraviolet detector at 254 nm. The limit of quantitation is 0.1 micrograms/ml (0.396 mumol/l for pirprofen and 0.400 mumol/l for the pyrrole derivative).
本文描述了一种同时测定人血浆中吡洛芬及其代谢物（吡咯衍生物）的方法。这两种化合物以及用作内标的吡咯衍生物的丁酸类似物用氯仿从血浆中萃取，然后反萃取到碱性缓冲液中。加酸后，水相用高效液相色谱法在254nm波长下使用固定波长紫外检测器进行测定。定量限为0.1微克/毫升（吡洛芬为0.396微摩尔/升，吡咯衍生物为0.400微摩尔/升）。
