Berger A E, Kuentzel S L, Davidson S O
J Immunol. 1985 Sep;135(3):1792-9.
The factors determining successful derivation of human T lymphocyte hybrids are largely unknown. This report describes diploid and tetraploid clones of the T cell line CEM which were fused with either a human T cell line (Jurkat) or with peripheral blood lymphocytes (PBL). Fusions of all CEMR clones with the Jurkat cell line yielded hybrids at a very high frequency (1 X 10(-4)). Fusion of diploid clones of CEM with PBL yielded no hybrids, whereas fusion of tetraploid clones of CEM with PBL resulted in growth frequencies of 1 to 3 X 10(-6). Enumeration of hybrids immediately after fusion indicated that in all cases, fused cells represented 5 to 10% of the population. That the ability to yield viable hybrids after fusion was a characteristic of tetraploid cells was indicated by the finding that tetraploid variants of a diploid clone could also yield viable hybrids after fusion. Possible mechanisms for the difference in results generated with diploid and tetraploid cells, and characteristics of the hybrid cells generated, are also discussed.
决定人类T淋巴细胞杂交体成功衍生的因素在很大程度上尚不清楚。本报告描述了T细胞系CEM的二倍体和四倍体克隆，它们与人类T细胞系（Jurkat）或外周血淋巴细胞（PBL）进行了融合。所有CEMR克隆与Jurkat细胞系的融合都以非常高的频率（1×10⁻⁴）产生杂交体。CEM二倍体克隆与PBL的融合未产生杂交体，而CEM四倍体克隆与PBL的融合产生了1至3×10⁻⁶的生长频率。融合后立即对杂交体进行计数表明，在所有情况下，融合细胞占群体的5%至10%。二倍体克隆的四倍体变体在融合后也能产生有活力的杂交体，这一发现表明融合后产生有活力杂交体的能力是四倍体细胞的一个特征。本文还讨论了二倍体细胞和四倍体细胞产生不同结果的可能机制，以及所产生杂交细胞的特征。
