Manheimer A J, Bona C A
Mech Ageing Dev. 1985 May 13;30(2):187-99. doi: 10.1016/0047-6374(85)90007-7.
The secondary and tertiary immune responses to TNP-KLH and the autoanti-immunoglobulin response were studied in 1-, 3- and 6-month-old 129/J and 129/Sv mice. A profound inability of aging 129/Sv mice (lifespan: 9 months) to produce gamma 2a anti-TNP antibodies relative to their normal counterparts was observed. However, this markedly low response could not be related to clearance of antibody by RFs since all 129 mice studied produced significant amounts of RFs after immunization with this T-dependent antigen. Furthermore, the results of the plaque forming cell assays indicated that young (1 month old) 129/Sv mice have an accelerated immune response while older mice produce significantly low direct and indirect responses compared to the control groups. The data suggest a fundamental dysfunction of B and/or T cells in 129/Sv mice and that spontaneous production of RFs in older mice may affect the ability of B and T cells to cooperate in a secondary response.
研究了129/J和129/Sv品系1、3和6月龄小鼠对三硝基苯-钥孔血蓝蛋白（TNP-KLH）的二次和三次免疫反应以及自身抗免疫球蛋白反应。观察到衰老的129/Sv小鼠（寿命：9个月）相对于正常同龄小鼠，产生γ2a抗TNP抗体的能力严重不足。然而，这种明显较低的反应与类风湿因子（RFs）清除抗体无关，因为所有研究的129小鼠在用这种T细胞依赖性抗原免疫后都产生了大量的RFs。此外，空斑形成细胞试验结果表明，年轻（1月龄）的129/Sv小鼠免疫反应加速，而与对照组相比，老年小鼠产生的直接和间接反应显著较低。数据表明129/Sv小鼠的B和/或T细胞存在基本功能障碍，并且老年小鼠中RFs的自发产生可能会影响B细胞和T细胞在二次反应中协同作用的能力。
