Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, PR China.
National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, 316022, PR China.
Fish Shellfish Immunol. 2024 Jul;150:109623. doi: 10.1016/j.fsi.2024.109623. Epub 2024 May 14.
The interleukin-12 (IL-12) family is a class of heterodimeric cytokines that play crucial roles in pro-inflammatory and pro-stimulatory responses. Although some IL-12 and IL-23 paralogues have been found in fish, their functional activity in fish remains poorly understood. In this study, Pf_IL-12p35a/b, Pf_IL-23p19 and Pf_IL-12p40a/b/c genes were cloned from yellow catfish (Pelteobagrus fulvidraco), four α-helices were found in Pf_IL-12p35a/b and Pf_IL-23p19. The transcripts of these six genes were relatively high in mucus and immune tissues of healthy individuals, and in gill leukocytes. Following Edwardsiella ictaluri infection, Pf_IL-12p35a/b and Pf_IL-23p19 mRNAs were induced in brain and kidney (or head kidney), Pf_IL-12p40a mRNA was induced in gill, and Pf_IL-12p40b/c mRNAs were induced in brain and liver (or skin). The mRNA expression of these genes in PBLs was induced by phytohaemagglutinin (PHA) and polyinosinic-polycytidylic acid (poly I:C), while lipopolysaccharides (LPS) induced the mRNA expression of Pf_IL-12p35a and Pf_IL-12p40b/c in PBLs. After stimulation with recombinant (r) Pf_IL-12 and rPf_IL-23 subunit proteins, either alone or in combination, mRNA expression patterns of genes related to T helper cell development exhibited distinct differences. The results suggest that Pf_IL-12 and Pf_IL-23 subunits may play important roles in regulating immune responses to pathogens and T helper cell development.
白细胞介素-12（IL-12）家族是一类异二聚体细胞因子，在促炎和促刺激反应中发挥关键作用。尽管在鱼类中发现了一些 IL-12 和 IL-23 旁系同源物，但它们在鱼类中的功能活性仍知之甚少。在这项研究中，从黄颡鱼（Pelteobagrus fulvidraco）中克隆了 Pf_IL-12p35a/b、Pf_IL-23p19 和 Pf_IL-12p40a/b/c 基因，Pf_IL-12p35a/b 和 Pf_IL-23p19 中发现了四个α-螺旋。这些六个基因的转录物在健康个体的黏液和免疫组织以及鳃白细胞中相对较高。在爱德华氏菌感染后，Pf_IL-12p35a/b 和 Pf_IL-23p19 mRNA 在脑和肾脏（或头肾）中被诱导，Pf_IL-12p40a mRNA 在鳃中被诱导，Pf_IL-12p40b/c mRNA 在脑和肝脏（或皮肤）中被诱导。这些基因在 PBLs 中的mRNA 表达被植物血凝素（PHA）和多聚肌苷酸-多聚胞苷酸（poly I:C）诱导，而脂多糖（LPS）诱导 PBLs 中 Pf_IL-12p35a 和 Pf_IL-12p40b/c 的 mRNA 表达。在用重组（r）Pf_IL-12 和 rPf_IL-23 亚基蛋白单独或组合刺激后，与 Th 细胞发育相关的基因的 mRNA 表达模式表现出明显的差异。结果表明，Pf_IL-12 和 Pf_IL-23 亚基可能在调节对病原体的免疫反应和 Th 细胞发育中发挥重要作用。
