蛋白质组学方法鉴定南印度泰米尔人中与胃癌相关的血清生物标志物
Proteomics Approach to Identify Serum Biomarkers Associated with Gastric Cancer in South Indian Tamils.
作者信息
Madhesh Jeevitha Chithra, Narasiman Manojkumar, Nagarajan Vignesh, Meenalosani S, Varshikaa Sweta, Sivagnanam Ananthi, Jayaraman Megala
机构信息
Department of Genetic Engineering, Faculty of Science and Technology, SRM Institute of Science and Technology, Kattankulathur, Chennai-603203, Tamilnadu, India.
Proteomics Department, Clinbiocare Technology, Tenkasi 627814, India.
出版信息
Comb Chem High Throughput Screen. 2025;28(7):1229-1239. doi: 10.2174/0113862073302521240429112034.
BACKGROUND
Identifying cancer-specific biomarkers is a crucial step in the disease screening process at a very early stage of tumor development. In recent years, Quantitative proteomic approaches have gained importance in identifying novel candidate markers in cancer. Gastric cancer has always been known as a life-threatening medical condition with high mortality rates.
OBJECTIVES
The objective of our research is to adapt serum samples from Indian gastric cancer patients to identify and understand the differentially regulated proteins in comparison with healthy individuals.
METHODS
A total of 30 serum isolates from gastric cancer patients and healthy individuals were obtained and subjected to 2-D Gel electrophoresis, and Tandem LC-MS analysis revealed 12 differentially expressed protein spots. The functional properties of identified proteins were further analyzed using PANTHER and STRING databases.
RESULTS
The differentially expressed protein spots were identified as three candidate proteins: Haptoglobin, Prohibitin, and Apolipoprotein. The protein interaction studies reveal that the haptoglobin fragments were upregulated, and the remaining two prohibitin and Apolipoprotein were down-regulated in gastric cancer patients.
CONCLUSION
All the proteins identified as biomarkers were found to be involved in regulating cell proliferation and stabilization of oxidative metabolism in the liver; therefore, differential regulation plays a crucial role in gastric cancer progression.
背景
在肿瘤发展的极早期阶段，识别癌症特异性生物标志物是疾病筛查过程中的关键步骤。近年来，定量蛋白质组学方法在识别癌症新候选标志物方面变得越来越重要。胃癌一直被认为是一种威胁生命的疾病，死亡率很高。
目的
我们研究的目的是采用印度胃癌患者的血清样本，与健康个体相比，识别并了解差异调节的蛋白质。
方法
共获得30份来自胃癌患者和健康个体的血清分离物，进行二维凝胶电泳，串联液相色谱-质谱分析揭示了12个差异表达的蛋白点。使用PANTHER和STRING数据库进一步分析已识别蛋白质的功能特性。
结果
差异表达的蛋白点被鉴定为三种候选蛋白：触珠蛋白、抑制素和载脂蛋白。蛋白质相互作用研究表明，在胃癌患者中触珠蛋白片段上调，而其余两种抑制素和载脂蛋白下调。
结论
所有被鉴定为生物标志物的蛋白质都被发现参与调节肝脏中的细胞增殖和氧化代谢稳定；因此，差异调节在胃癌进展中起关键作用。
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