Subbannayya Yashwanth, Mir Sartaj Ahmad, Renuse Santosh, Manda Srikanth S, Pinto Sneha M, Puttamallesh Vinuth N, Solanki Hitendra Singh, Manju H C, Syed Nazia, Sharma Rakesh, Christopher Rita, Vijayakumar M, Veerendra Kumar K V, Keshava Prasad T S, Ramaswamy Girija, Kumar Rekha V, Chatterjee Aditi, Pandey Akhilesh, Gowda Harsha
Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; Rajiv Gandhi University of Health Sciences, Bangalore 560041, Karnataka, India; Department of Biochemistry, Kidwai Memorial Institute of Oncology, Bangalore 560029, Karnataka, India.
Institute of Bioinformatics, International Technology Park, Bangalore 560066, India; Manipal University, Manipal 576 104, Karnataka, India.
J Proteomics. 2015 Sep 8;127(Pt A):80-8. doi: 10.1016/j.jprot.2015.04.021. Epub 2015 May 5.
Gastric adenocarcinoma is an aggressive cancer with poor prognosis. Blood based biomarkers of gastric cancer have the potential to improve diagnosis and monitoring of these tumors. Proteins that show altered levels in the circulation of gastric cancer patients could prove useful as putative biomarkers. We used an iTRAQ-based quantitative proteomic approach to identify proteins that show altered levels in the sera of patients with gastric cancer. Our study resulted in identification of 643 proteins, of which 48 proteins showed increased levels and 11 proteins showed decreased levels in serum from gastric cancer patients compared to age and sex matched healthy controls. Proteins that showed increased expression in gastric cancer included inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), Mannose-binding protein C (MBL2), sex hormone-binding globulin (SHBG), insulin-like growth factor-binding protein 2 (IGFBP2), serum amyloid A protein (SAA1), Orosomucoid 1 (ORM1) and extracellular superoxide dismutase [Cu-Zn] (SOD3). We used multiple reaction monitoring assays and validated elevated levels of ITIH4 and SAA1 proteins in serum from gastric cancer patients.
Gastric cancer is a highly aggressive cancer associated with high mortality. Serum-based biomarkers are of considerable interest in diagnosis and monitoring of various diseases including cancers. Gastric cancer is often diagnosed at advanced stages resulting in poor prognosis and high mortality. Pathological diagnosis using biopsy specimens remains the gold standard for diagnosis of gastric cancer. Serum-based biomarkers are of considerable importance as they are minimally invasive. In this study, we carried out quantitative proteomic profiling of serum from gastric cancer patients to identify proteins that show altered levels in gastric cancer patients. We identified more than 50 proteins that showed altered levels in gastric cancer patient sera. Validation in a large cohort of well classified patient samples would prove useful in identifying novel blood based biomarkers for gastric cancers. This article is part of a Special Issue entitled: Proteomics in India.
胃腺癌是一种侵袭性癌症，预后较差。胃癌的血液生物标志物有潜力改善这些肿瘤的诊断和监测。在胃癌患者循环中水平发生改变的蛋白质可能被证明是有用的假定生物标志物。我们使用基于iTRAQ的定量蛋白质组学方法来鉴定在胃癌患者血清中水平发生改变的蛋白质。我们的研究鉴定出643种蛋白质，其中与年龄和性别匹配的健康对照相比，48种蛋白质在胃癌患者血清中的水平升高，11种蛋白质的水平降低。在胃癌中表达增加的蛋白质包括α-胰蛋白酶抑制剂重链H4（ITIH4）、甘露糖结合蛋白C（MBL2）、性激素结合球蛋白（SHBG）、胰岛素样生长因子结合蛋白2（IGFBP2）、血清淀粉样A蛋白（SAA1）、类粘蛋白1（ORM1）和细胞外超氧化物歧化酶[铜-锌]（SOD3）。我们使用多反应监测分析并验证了胃癌患者血清中ITIH4和SAA1蛋白水平升高。
胃癌是一种高度侵袭性的癌症，死亡率高。基于血清的生物标志物在包括癌症在内的各种疾病的诊断和监测中备受关注。胃癌通常在晚期被诊断出来，导致预后不良和高死亡率。使用活检标本进行病理诊断仍然是胃癌诊断的金标准。基于血清的生物标志物非常重要，因为它们具有微创性。在本研究中，我们对胃癌患者的血清进行了定量蛋白质组分析，以鉴定在胃癌患者中水平发生改变的蛋白质。我们鉴定出50多种在胃癌患者血清中水平发生改变的蛋白质。在大量分类良好的患者样本中进行验证将有助于鉴定新型的基于血液的胃癌生物标志物。本文是名为：印度蛋白质组学的特刊的一部分。
