微流控 3D 细胞毒性分析

Microfluidic 3D Cytotoxic Assay.

机构信息

Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, South Korea.

Department of Mechanical Engineering, Seoul National University, Seoul, South Korea.

出版信息

Methods Mol Biol. 2024;2804:209-221. doi: 10.1007/978-1-0716-3850-7_13.

DOI:10.1007/978-1-0716-3850-7_13

PMID:38753150

Abstract

Microfluidic-based cytotoxic assays provide high physiological relevance with the potential to replace conventional animal experiments and two-dimensional (2D) assays. Here, a 3D method utilizing a microfluidic platform for analysis of lymphocyte cytotoxicity is introduced in detail, including platform design, cell culture method, real-time cytotoxic assay setup, and image-based analysis. A 2D experimental method is used for comparison, which effectively demonstrates the advantages of 3D microfluidic platforms in closely recapitulating immune responses within the tumor microenvironment. Moreover, a wide range of experimental possibilities and applications using microfluidic 3D cytotoxic assays is introduced in this chapter, along with their capabilities, limitations, and future outlook.

摘要

基于微流控的细胞毒性分析提供了高度的生理相关性，具有替代传统动物实验和二维（2D）分析的潜力。本文详细介绍了一种利用微流控平台分析淋巴细胞细胞毒性的 3D 方法，包括平台设计、细胞培养方法、实时细胞毒性分析设置和基于图像的分析。同时还使用了一种 2D 实验方法进行比较，有效地证明了 3D 微流控平台在紧密重现肿瘤微环境中的免疫反应方面的优势。此外，本章还介绍了微流控 3D 细胞毒性分析的广泛实验可能性和应用，以及它们的功能、局限性和未来展望。