Tan J A, Tsay S S
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1985 Feb;18(1):44-53.
A thermostable alpha-amylase producing bacterium has been isolated from soil and identified as Bacillus licheniformis B7. Cultural conditions of this strain for alpha-amylase production were studied. The optimum temperature and incubation period for enzyme production were 50 degrees C and 7 days respectively. Starch with a concentration of 2.5% was the best carbon source for enzyme production. Besides the above conditions, the addition of 0.05% FeCl3 X 6H2O, 0.6% yeast extract, 0.35% peptone, 0.2% Na-citrate and 0.008% CaCl2 X 2H2O to the growth medium produced maximum enzyme activity. The alpha-amylase produced by this strain showed maximal activity at 90 degrees C and pH 9.0.
从土壤中分离出一株产耐热α-淀粉酶的细菌，鉴定为地衣芽孢杆菌B7。研究了该菌株产α-淀粉酶的培养条件。产酶的最适温度和培养时间分别为50℃和7天。浓度为2.5%的淀粉是产酶的最佳碳源。除上述条件外，在生长培养基中添加0.05%的FeCl3·6H2O、0.6%的酵母提取物、0.35%的蛋白胨、0.2%的柠檬酸钠和0.008%的CaCl2·2H2O可产生最大酶活性。该菌株产生的α-淀粉酶在90℃和pH 9.0时表现出最大活性。
