Tsubouchi S, Potten C S
Int J Radiat Biol Relat Stud Phys Chem Med. 1985 Sep;48(3):361-9. doi: 10.1080/09553008514551361.
Epithelial cell recruitment was examined in mouse ileum after external gamma-irradiation (50 cGy) or internal beta-irradiation (0.148 MBq/g of [3H]thymidine), using the per cent-labelled-mitoses method and by analysing the distribution of mitotic cells in the crypts. In the presumptive stem cell zone at the lower cell positions of the crypt, the slowly cycling cells decreased their cell cycle 6 or 12 hours after a dose of 50 cGy. In the higher cell positions, a slight shortening of the cell cycle was also observed. After administration of a high dose of [3H]thymidine, dormant (G0) cells also entered the cell cycle in the lower cell positions. The results suggest that stem cells in the crypt may react to irradiation in two ways: first, by shortening the cell cycle in cycling cells; secondly, by an entry into the cell cycle by other dormant cells. There was destruction of some cycling stem cells before any recruitment. The data support the idea that the stem cell population in the crypt is heterogeneous.
采用标记有丝分裂百分数法并通过分析隐窝中有丝分裂细胞的分布情况，对经外部γ射线照射（50 cGy）或内部β射线照射（0.148 MBq/g的[³H]胸腺嘧啶核苷）后的小鼠回肠中的上皮细胞募集情况进行了检测。在隐窝较低细胞位置的假定干细胞区，剂量为50 cGy后6或12小时，慢周期细胞缩短了其细胞周期。在较高细胞位置，也观察到细胞周期略有缩短。给予高剂量的[³H]胸腺嘧啶核苷后，处于静止期（G0期）的细胞也在较低细胞位置进入细胞周期。结果表明，隐窝中的干细胞可能以两种方式对辐射作出反应：第一，通过缩短周期中细胞的细胞周期；第二，通过其他静止细胞进入细胞周期。在任何募集之前，一些周期中的干细胞就已遭到破坏。这些数据支持隐窝中干细胞群体是异质性的这一观点。
