Department of Molecular Medicine, Division of Physiology, Institute of Basic Medical Science, University of Oslo, Oslo, Norway.
Department of Research and Development, Division of Emergencies and Critical Care, Oslo University Hospital Ullevål, Oslo, Norway.
Scand Cardiovasc J. 2024 May 9;58(1):2353070. doi: 10.1080/14017431.2024.2353070. Epub 2024 May 17.
The role of diabetes mellitus as a risk factor for the development of calcific aortic valve disease has not been fully clarified. Aortic valve interstitial cells (VICs) have been suggested to be crucial for calcification of the valve. Induced calcification in cultured VICs is a good model for aortic valve calcification. The purpose of this study was to investigate whether increased glucose levels increase experimentally induced calcification in cultured human VICs. VICs were isolated from explanted calcified aortic valves after valve replacement. Osteogenic medium induced calcification of cultured VICs at different glucose levels (5, 15, and 25 mM). Calcium deposits were visualized using Alizarin Red staining and measured spectrophotometrically. The higher the glucose concentration, the lower the level of calcification. High glucose (25 mM) reduced calcification by 52% compared with calcification at a physiological (5 mM) glucose concentration (correlation and regression analysis: = -0.55, = .025 with increased concentration of glucose). hyperglycemia-like conditions attenuated calcification in VICs. High glucose levels may trigger a series of events that secondarily stimulate calcification of VICs .
糖尿病在钙化性主动脉瓣疾病的发展中作为一个风险因素的作用尚未完全阐明。主动脉瓣间质细胞（VICs）被认为对瓣膜钙化至关重要。培养的 VICs 中的诱导钙化是主动脉瓣钙化的良好模型。本研究旨在探讨高血糖是否会增加培养的人 VICs 中实验性诱导的钙化。VICs 从瓣膜置换后钙化的主动脉瓣中分离出来。在不同葡萄糖水平（5、15 和 25 mM）的成骨培养基中诱导培养的 VIC 钙化。使用茜素红染色可视化钙沉积并通过分光光度法测量。葡萄糖浓度越高，钙化水平越低。与生理（5 mM）葡萄糖浓度相比，高葡萄糖（25 mM）使钙化减少了 52%（相关和回归分析：r = -0.55，p =.025，葡萄糖浓度增加）。高血糖样条件可减轻 VIC 中的钙化。高血糖水平可能引发一系列继发于 VIC 钙化的事件。
