Deitch E A
Surgery. 1985 Sep;98(3):587-93.
Controversy exists over whether lymphocyte mitogenic activity decreases after thermal injury, as well as whether the decreased activity is of clinical significance. Therefore to characterize the pattern of postburn lymphocyte dysfunction, the rates of the patient's spontaneous (SBT) and stimulated (mitogen phytohemagglutinin) lymphocyte blastogenesis were measured and correlated with the biologic effect of the patient's serum and lymphokines on control mononuclear cell blastogenesis. The SBT increased after thermal injury (p less than 0.01) in contrast to the mitogenic-induced blastogenic response, which was either normal or decreased depending on the mechanics of data analysis. The level of SBT elevation but not stimulated lymphocyte activity would identify patients who had sepsis (p less than 0.05). The changes in lymphocyte activity could not be explained by the presence of circulating serum mediators or the biologic effect of soluble mononuclear cell products (lymphokines). The results of these experiments clearly document that after thermal injury lymphocytes are being activated in vivo even when the patient's serum is suppressive and the phytohemagglutinin-stimulated activity is depressed. Therefore the level of SBT must be taken into consideration when lymphocyte function is evaluated after thermal injury.
关于热损伤后淋巴细胞有丝分裂活性是否降低以及这种降低的活性是否具有临床意义存在争议。因此，为了描述烧伤后淋巴细胞功能障碍的模式，测量了患者自发（SBT）和受刺激（有丝分裂原植物血凝素）淋巴细胞增殖的速率，并将其与患者血清和淋巴因子对对照单核细胞增殖的生物学效应相关联。与有丝分裂诱导的增殖反应相反，热损伤后SBT增加（p<0.01），有丝分裂诱导的增殖反应根据数据分析方法的不同而正常或降低。SBT升高的水平而非受刺激淋巴细胞的活性可识别患有败血症的患者（p<0.05）。淋巴细胞活性的变化无法用循环血清介质的存在或可溶性单核细胞产物（淋巴因子）的生物学效应来解释。这些实验结果清楚地表明，热损伤后即使患者血清具有抑制作用且植物血凝素刺激的活性降低，淋巴细胞仍在体内被激活。因此，热损伤后评估淋巴细胞功能时必须考虑SBT水平。
