Department of Microbiology, University of Jhang, Jhang, Pakistan.
Department of Biosciences, COMSATS University (CUI), Park Road, Tarlai Kalan, Chak Shahzad, Islamabad,45550, Pakistan.
Diagn Microbiol Infect Dis. 2024 Aug;109(4):116346. doi: 10.1016/j.diagmicrobio.2024.116346. Epub 2024 May 9.
Rotaviruses belong to genotype VP4-P[8] are a significant cause of severe loose diarrhea in infants and young children. In the present study, we characterised the complete genome of three of the Pakistani P[8]b RVA strains by Illumina HiSeq sequencing technology to determine the complete genotype constellation providing insight into the evolutionary dynamics of their genes using maximum likelihood analysis. The maximum genomic sequences of our study strains were similar to more recent human Wa-Like G1P[8]a, G3P[8]a, G4P[6], G4P[8], G9P[4], G9P[8]a, G11P[25],G12P[8]a and G12P[6] strains circulating around the world. Therefore, strains PAK274, PAK439 and PAK624 carry natively distinctive VP4 gene with universally common human Wa-Like genetic backbone. Comparing our study P[8]b strains with vaccines strains Rotarix and RotaTeq, multiple amino acid differences were examined between vaccine virus antigenic epitopes and Pakistani isolates. Over time, these differences may result in the selection for strains that will escape the vaccine-induced RVA-neutralizing-antibody effect.
轮状病毒属于 VP4-P[8]基因型，是导致婴幼儿严重腹泻的重要原因。本研究采用 Illumina HiSeq 测序技术对三株巴基斯坦 P[8]b RVA 株进行了全基因组测序，通过最大似然法分析明确了其完整的基因型构成，从而深入了解了它们基因的进化动态。本研究中病毒株的最大基因组序列与最近流行的 Wa 样 G1P[8]a、G3P[8]a、G4P[6]、G4P[8]、G9P[4]、G9P[8]a、G11P[25]、G12P[8]a 和 G12P[6] 人类株相似。因此，PAK274、PAK439 和 PAK624 株天然携带具有普遍人类 Wa 样遗传背景的独特 VP4 基因。将本研究中的 P[8]b 株与 Rotarix 和 RotaTeq 疫苗株进行比较，发现疫苗病毒抗原表位与巴基斯坦分离株之间存在多个氨基酸差异。随着时间的推移，这些差异可能导致能够逃避疫苗诱导的轮状病毒中和抗体效应的病毒株被选择出来。
