Hønberg P Z
Acta Pathol Microbiol Immunol Scand B. 1985 Jun;93(3):175-9. doi: 10.1111/j.1699-0463.1985.tb02873.x.
In order to obtain a way of subtyping Haemophilus influenzae type b strains, outer-membrane protein patterns were investigated. Outer-membrane proteins from 45 different Haemophilus influenzae type b strains were examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The non-heated sarkosyl insoluble outer-membrane preparations all contained four major proteins with molecular weights (Mr) of 39,000, 33,000, 30,000, and 28,000, except for one strain which possessed a protein weighing approximately 32,000. After heating the samples a 45,000 Mr protein appeared in 43 of the strains, and a 46,000 Mr in two. The 39,000 Mr protein remained unchanged, whereas the 33,000 Mr protein disappeared. The 30,000 and the 28,000 Mr proteins became more clearly separated. A faint 16,000 Mr band was observed in both heated and non-heated samples. 42 strains with the light heat-modified protein band had identical protein patterns and could not be subdivided by the methods used and may therefore constitute a clone. The remaining strains had individual patterns.
为了获得一种对b型流感嗜血杆菌菌株进行分型的方法，对其外膜蛋白模式进行了研究。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳对45株不同的b型流感嗜血杆菌菌株的外膜蛋白进行了检测。除了一株拥有一种分子量约为32,000的蛋白的菌株外，未加热的 Sarkosyl 不溶性外膜制剂均含有四种主要蛋白，其分子量（Mr）分别为39,000、33,000、30,000和28,000。加热样品后，43株菌株中出现了一种分子量为45,000的蛋白，两株中出现了一种分子量为46,000的蛋白。分子量为39,000的蛋白保持不变，而分子量为33,000的蛋白消失。分子量为30,000和28,000的蛋白分离得更明显。在加热和未加热的样品中均观察到一条 faint 16,000 Mr 的条带。42株具有轻度热修饰蛋白条带的菌株具有相同的蛋白模式，无法用所使用的方法进一步细分，因此可能构成一个克隆。其余菌株具有各自的模式。
