Elliott J A, Bosley G S, Carlone G M, Plikaytis B D, Facklam R R
J Clin Microbiol. 1987 Aug;25(8):1476-80. doi: 10.1128/jcm.25.8.1476-1480.1987.
Outer membrane proteins (OMPs) were prepared from 20 previously established subtypes of Haemophilus influenzae type b. Proteins were separated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (11% acrylamide). Each lane in the gels contained two internal molecular weight standards. One central lane contained a range of molecular weight standards, which were used to calculate a molecular weight curve for each gel. Migration distances of the OMPs were determined with a soft laser-scanning densitometer, and the distances were normalized by using the mean migration distances of the internal standards. The protein patterns of all subtypes were compared by a recently described method (B.D. Plikaytis, G.M. Carlone, and B.B. Plikaytis, J. Gen. Microbiol. 132:2653-2660, 1986). All subtypes could be differentiated by this method. The ability to store and compare numerous OMP patterns from different isolates of H. influenzae type b, separated with a single homogeneous polyacrylamide gel, will facilitate the continued development of a subtyping system based on these proteins.
从先前确定的20种b型流感嗜血杆菌亚型中制备外膜蛋白（OMPs）。蛋白质通过一维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（11%丙烯酰胺）进行分离。凝胶中的每条泳道都包含两个内部分子量标准品。中间的一条泳道包含一系列分子量标准品，用于计算每张凝胶的分子量曲线。用软激光扫描密度计测定OMPs的迁移距离，并以内标物的平均迁移距离对距离进行标准化。通过最近描述的一种方法（B.D.普利卡蒂斯、G.M.卡洛内和B.B.普利卡蒂斯，《普通微生物学杂志》132:2653 - 2660，1986年）比较所有亚型的蛋白质图谱。通过这种方法可以区分所有亚型。利用单一均匀聚丙烯酰胺凝胶分离来自不同b型流感嗜血杆菌分离株的众多OMP图谱并进行存储和比较的能力，将有助于基于这些蛋白质的分型系统的持续发展。
