College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, China.
Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China.
BMC Plant Biol. 2024 May 17;24(1):409. doi: 10.1186/s12870-024-05134-0.
Bletilla striata (Thunb.) Reichb. f. (B. striata) is a perennial herbaceous plant in the Orchidaceae family known for its diverse pharmacological activities, such as promoting wound healing, hemostasis, anti-inflammatory effects, antioxidant properties, and immune regulation. Nevertheless, the microbe-plant-metabolite regulation patterns for B. striata remain largely undetermined, especially in the field of rhizosphere microbes. To elucidate the interrelationships between soil physics and chemistry and rhizosphere microbes and metabolites, a comprehensive approach combining metagenome analysis and targeted metabolomics was employed to investigate the rhizosphere soil and tubers from four provinces and eight production areas in China.
Our study reveals that the core rhizosphere microbiome of B. striata is predominantly comprised of Paraburkholderia, Methylibium, Bradyrhizobium, Chitinophaga, and Mycobacterium. These microbial species are recognized as potentially beneficial for plants health. Comprehensive analysis revealed a significant association between the accumulation of metabolites, such as militarine and polysaccharides in B. striata and the composition of rhizosphere microbes at the genus level. Furthermore, we found that the soil environment indirectly influenced the metabolite profile of B. striata by affecting the composition of rhizosphere microbes. Notably, our research identifies soil organic carbon as a primary driving factor influencing metabolite accumulation in B. striata.
Our fndings contribute to an enhanced understanding of the comprehensive regulatory mechanism involving microbe-plant-metabolite interactions. This research provides a theoretical basis for the cultivation of high-quality traditional Chinese medicine B. striata.
白及（Bletilla striata (Thunb.) Reichb. f.）是兰科白及属的多年生草本植物，具有多种药理活性，如促进伤口愈合、止血、抗炎作用、抗氧化特性和免疫调节。然而，白及的微生物-植物-代谢物调控模式在很大程度上仍未确定，特别是在根际微生物领域。为了阐明土壤物理化学性质与根际微生物和代谢物之间的相互关系，本研究采用宏基因组分析和靶向代谢组学相结合的综合方法，对来自中国四个省和八个产区的白及根际土壤和块茎进行了研究。
本研究表明，白及的核心根际微生物组主要由 Paraburkholderia、Methylibium、Bradyrhizobium、Chitinophaga 和 Mycobacterium 组成。这些微生物物种被认为对植物健康有益。综合分析表明，白及中代谢物的积累（如千里光菲宁碱和多糖）与根际微生物属水平的组成之间存在显著关联。此外，我们发现土壤环境通过影响根际微生物的组成间接影响白及的代谢物谱。值得注意的是，本研究确定土壤有机碳是影响白及代谢物积累的主要驱动因素。
本研究结果有助于深入了解微生物-植物-代谢物相互作用的综合调控机制。本研究为高质量传统中药白及的栽培提供了理论依据。
