革兰氏阴性菌脂多糖O抗原的放射免疫测定：抗原溶解度的影响
Radioimmunoassay for Gram-negative bacterial lipopolysaccharide O antigens: influence of antigen solubility.
作者信息
Munford R S, Hall C L
出版信息
Infect Immun. 1979 Oct;26(1):42-8. doi: 10.1128/iai.26.1.42-48.1979.
Abstract
We have developed a solid-phase radioimmunoassay technique for specific gram-negative bacterial lipopolysaccharide (LPS) O antigens. The method exploits the high-titer, specific immunoglobulin M response of the rabbit to LPS immunization to measure as little as 5 ng of homologous LPS per ml with less than 0.5% cross-reactivity toward heterologous LPS or culture supernatants. We found that O antigen in complete LPS was less available for antibody binding than O antigen in the soluble polysaccharide derived by mild acid hydrolysis of LPS and that triethylamine-induced disaggregation of complete LPS increased its activity in the assay. Quantitation of O antigen with the assay was thus influenced by the physical state of LPS or "free" O antigen.
摘要
我们已经开发出一种用于检测特定革兰氏阴性菌脂多糖（LPS）O抗原的固相放射免疫分析技术。该方法利用兔子对LPS免疫产生的高效价、特异性免疫球蛋白M反应，每毫升可检测低至5纳克的同源LPS，对异源LPS或培养上清液的交叉反应性低于0.5%。我们发现，完整LPS中的O抗原与通过LPS温和酸水解得到的可溶性多糖中的O抗原相比，与抗体结合的能力较弱，并且三乙胺诱导的完整LPS解聚增加了其在分析中的活性。因此，用该分析方法对O抗原的定量受到LPS或“游离”O抗原物理状态的影响。
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