College of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil.
Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR, USA.
Primates. 2024 Jul;65(4):341-353. doi: 10.1007/s10329-024-01136-6. Epub 2024 May 18.
There are more than 200 species and subspecies of Neotropical Primates of which more than 40% are listed as threatened by the IUCN Red List of Threatened Species. Both in situ and ex situ conservation programs can benefit from the use of assisted reproductive technologies. The objective of this study was to evaluate, for the first time, cryopreservation techniques for Alouatta caraya semen. Semen samples were collected from five adult males, analyzed, and frozen in either Test-egg yolk or Test-soy lecithin-based extenders containing either 3 or 4% glycerol. Frozen-thawed samples were analyzed at 10, 40, and 80 min post-thaw. Egg yolk-based extenders were overall better than soy lecithin-based extenders. There was no significant difference between 3 and 4% glycerol in any of the parameters analyzed, however, 4% glycerol in egg yolk-based extender produced more favorable results for total motility, intact plasma membrane, lipid peroxidation, and DNA fragmentation index. This study brought novel information on semen characteristics and cryopreservation aspects for A. caraya, which can help shape future experiments to improve the outcome of frozen-thawed sperm for this and other species of Neotropical primates.
新热带灵长类动物有 200 多种，其中超过 40%被列入 IUCN 红色名录濒危物种。原地和异地保护计划都可以受益于辅助生殖技术的使用。本研究的目的是首次评估针对新世界猴（Alouatta caraya）精液的冷冻保存技术。从五只成年雄性个体中采集精液样本，进行分析，并在含有 3%或 4%甘油的基于测试卵黄或测试大豆卵磷脂的两种扩展剂中进行冷冻。冷冻-解冻样本在解冻后 10、40 和 80 分钟进行分析。基于卵黄的扩展剂总体上优于基于大豆卵磷脂的扩展剂。在任何分析参数中，3%和 4%甘油之间均无显著差异，但在基于卵黄的扩展剂中添加 4%甘油可使总活力、完整质膜、脂质过氧化和 DNA 片段化指数产生更有利的结果。本研究为 A. caraya 的精液特征和冷冻保存方面提供了新的信息，这有助于为该物种和其他新世界灵长类动物的冷冻精液制定未来的实验方案，以提高冷冻-解冻精子的效果。
