Fang Yan, Lin Yukai, Wang Linyu, Zheng Xinwei, Chen Qinhui, Weng Yunxiang, Liu Haiqing
College of Chemistry and Materials Science, Fujian Normal University, Fujian 350007, China.
College of Chemistry and Materials Science, Fujian Normal University, Fujian 350007, China.
Int J Biol Macromol. 2024 Jun;270(Pt 2):132440. doi: 10.1016/j.ijbiomac.2024.132440. Epub 2024 May 16.
Hemostatic powder is widely utilized in emergency situations to control bleeding due to its ability to work well on wounds with irregular shapes, ease of application, and long-term stability. However, traditional powder often suffers from limited tissue adhesion and insufficient support for blood clot formation, leaving it susceptible to displacement by the flow of blood. This study introduces a hemostatic powder composed of tannic modified mesoporous bioactive glass (TMBG), cationic quaternized chitosan (QCS), and anionic hyaluronic acid modified with catechol group (HADA). The resulting TMBG/QCS/HADA based hemostatic powder (TMQH) rapidly absorbs plasma, concentrating blood coagulation factors. Simultaneously, the water-soluble QCS and HADA interact to form a 3D network structure, which can be strengthened by crosslinking with TMBG. This network effectively captures clustered blood coagulation factors, leading to a strong and adhesive thrombus that resists disruption from blood flow. TMQH exhibits superior efficacy in promoting hemostasis compared to Celox™ both in rat arterial injuries and non-compressible liver puncture wounds. TMQH demonstrates excellent antibacterial activity, cytocompatibility, and blood compatibility. These outstanding superiorities in blood clotting capability, wet tissue adhesion, antibacterial activity, safety for living organisms, ease of application, and long-term stability, make TMQH highly suitable for emergency hemostasis.
止血粉因其在不规则形状伤口上效果良好、易于应用且具有长期稳定性，而被广泛用于紧急止血情况。然而，传统粉末通常存在组织粘附性有限以及对血凝块形成的支持不足的问题，容易因血流而移位。本研究介绍了一种由单宁酸改性的介孔生物活性玻璃（TMBG）、阳离子季铵化壳聚糖（QCS）和经儿茶酚基团改性的阴离子透明质酸（HADA）组成的止血粉。由此得到的基于TMBG/QCS/HADA的止血粉（TMQH）能迅速吸收血浆，浓缩凝血因子。同时，水溶性的QCS和HADA相互作用形成三维网络结构，该结构可通过与TMBG交联得到强化。这种网络有效地捕获聚集的凝血因子，形成坚固且有粘附性的血栓，抵抗血流的破坏。在大鼠动脉损伤和不可压缩肝脏穿刺伤口模型中，TMQH在促进止血方面均表现出优于Celox™的效果。TMQH具有出色的抗菌活性、细胞相容性和血液相容性。在凝血能力、湿组织粘附性、抗菌活性、对生物体的安全性、易于应用以及长期稳定性等方面的这些突出优势，使得TMQH非常适合用于紧急止血。
