A serum independent medium effective in all aspects of hybridoma technology and immunological applications.

KC 2000 TM is a serum-free medium designed and developed for use in all phases of hybridoma technology providing maximum growth and antibody production. This unique medium provides consistent results as its performance is not affected by variation found in serum. In addition, the use of KC 2000 TM reduces the risk of contamination of cultures by mycoplasma and viruses which may be present in low concentrations in serum. Antibody production and assays are performed in KC 2000 TM without high concentrations of interfering proteins. Only those proteins necessary to produce maximum performance in all hybridoma applications are present (250 micrograms protein/ml). Extensive testing has shown that this serum-free medium yields maximum results in all aspects of hybridoma technology, including the generation of antibody secreting hybridomas, as well as their subcloning. With a single exception, all cell lines tested could be transferred directly from medium containing serum into KC 2000 TM. Consistent growth and antibody production was maintained during subsequent passaging of these cells. These results show that KC 2000 TM is unique among serum-free media as it is the only serum-free medium that can be successfully utilized in the selection of HAT-resistant hybrid cell clones after use of a single type of medium throughout the entire process of development and maintenance of hybridoma cell lines.