Center for Nanotechnology in Drug Delivery, Shiraz University of Medical Sciences, Shiraz 7146864685, Iran.
Student Research Committee, Shiraz University of Medical Sciences, Shiraz 7146864685, Iran.
J Pharm Pharmacol. 2024 Aug 2;76(8):995-1005. doi: 10.1093/jpp/rgae046.
Milk thistle has long been used in the treatment of liver and biliary disorders. In the present study, to make a long-acting delivery system for silibinin (SBN, a major active constituent of milk thistle seeds with antioxidant and hepatoprotective function), mesoporous silica composite nanoparticles (NC) were synthesized and coated with RBC membrane.
A modified Stöber method was used for NC synthesis, which was then characterized using FE-SEM, DLS, TEM, FTIR, and EDAX techniques. A suitable lysis buffer was used to prepare RBC-ghost, and sonication was used to coat SBN-loaded NC (SBN-NC). The RBC-ghost coated SBN-NC (SBN-NC-RBCG) was evaluated by SDS-PAGE, Bradford, TEM, EDAX, and DLS methods. SBN release was then compared for the SBN-NC and SBN-NC-RBCG samples.
the RBC membrane proteins were recovered from the coating of SBN-NC-RBCG, and SBN release was sustained over 24 h when compared with the SBN-NC.
Overall, through prolonging circulation in the bloodstream and evading the immune system, the developed system can improve SBN bioavailability in liver inflammation and fibrosis conditions that need further research.
水飞蓟素长期以来一直被用于治疗肝脏和胆道疾病。在本研究中，为了制备水飞蓟宾（SBN，一种具有抗氧化和保肝作用的水飞蓟种子的主要活性成分）的长效递药系统，合成了介孔硅复合纳米粒（NC）并包覆红细胞膜。
采用改良的Stöber 法合成 NC，并用 FE-SEM、DLS、TEM、FTIR 和 EDAX 技术进行表征。使用合适的裂解缓冲液制备 RBC-ghost，并用超声处理包覆载有 SBN 的 NC（SBN-NC）。通过 SDS-PAGE、Bradford、TEM、EDAX 和 DLS 方法评价包覆 RBC-ghost 的 SBN-NC（SBN-NC-RBCG）。然后比较 SBN-NC 和 SBN-NC-RBCG 样品的 SBN 释放情况。
从 SBN-NC-RBCG 的包覆中回收了 RBC 膜蛋白，与 SBN-NC 相比，SBN 的释放可持续 24 小时以上。
总之，通过延长在血液中的循环时间并逃避免疫系统，开发的系统可以提高在需要进一步研究的肝脏炎症和纤维化情况下 SBN 的生物利用度。
