College of Urban and Environmental Science, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Earth Surface System and Environment Carrying Capacity, Xi'an 710127, China.
College of Urban and Environmental Science, Northwest University, Xi'an 710127, China.
Bioresour Technol. 2024 Jul;403:130857. doi: 10.1016/j.biortech.2024.130857. Epub 2024 May 17.
Immobilization technology is a promising way to improve effectiveness and stability of microbial remediation for polycyclic aromatic hydrocarbons (PAHs), in which carrier material is one of key factors restricting removal efficiency. In this study, fulvic acid-wheat straw biochar (FA/WS) composites were applied for immobilization of an efficient PAHs degrading bacterium Stenotrophomonas maltophilia (SPM). FA/WS&SPM showed superior degradation capacity than free bacteria and biochar-immobilized bacteria, with the removal efficiency of pyrene (20 mg L) reaching 90.5 % (7 days). Transcriptome analysis revealed that FA in the carrier materials can promote transportation and degradation of pyrene, and cell growth, as well as inhibit cell apoptosis. Enzyme activity and degradation products detection showed that SPM utilized both phthalic acid and salicylic acid metabolic pathways to degrade pyrene. Practicality of FA/WS&SPM for different kinds of PAHs remediation had been verified in contaminated soil, demonstrating a great potential in the field of PAHs polluted sites remediation.
固定化技术是提高微生物修复多环芳烃（PAHs）效果和稳定性的一种有前途的方法，其中载体材料是限制去除效率的关键因素之一。本研究应用富里酸-麦草生物炭（FA/WS）复合材料固定高效多环芳烃降解菌寡养单胞菌（SPM）。FA/WS&SPM 表现出比游离菌和生物炭固定菌更高的降解能力，对芘（20mg/L）的去除效率达到 90.5%（7 天）。转录组分析表明，载体材料中的 FA 可以促进菲的运输和降解，以及细胞生长，并抑制细胞凋亡。酶活性和降解产物检测表明，SPM 利用邻苯二甲酸和水杨酸代谢途径来降解芘。在污染土壤中验证了 FA/WS&SPM 对不同类型 PAHs 修复的实用性，为 PAHs 污染场地修复领域提供了巨大潜力。
