Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI, USA.
Biochim Biophys Acta Mol Cell Res. 2024 Aug;1871(6):119749. doi: 10.1016/j.bbamcr.2024.119749. Epub 2024 May 17.
The role of IscR in regulating the transcription of genes involved in Fe-S cluster homeostasis has been well established for the model organism Escherichia coli K12. In this bacterium, IscR coordinates expression of the Isc and Suf Fe-S cluster assembly pathways to meet cellular Fe-S cluster demands shaped by a variety of environmental cues. However, since its initial discovery nearly 25 years ago, there has been growing evidence that IscR function extends well beyond Fe-S cluster homeostasis, not only in E. coli, but in bacteria of diverse lifestyles. Notably, pathogenic bacteria have exploited the ability of IscR to respond to changes in oxygen tension, oxidative and nitrosative stress, and iron availability to navigate their trajectory in their respective hosts as changes in these cues are frequently encountered during host infection. In this review, we highlight these broader roles of IscR in different cellular processes and, in particular, discuss the importance of IscR as a virulence factor for many bacterial pathogens.
IscR 在调节涉及 Fe-S 簇动态平衡的基因转录中的作用已在模式生物大肠杆菌 K12 中得到充分证实。在该细菌中，IscR 协调 Isc 和 Suf Fe-S 簇组装途径的表达，以满足各种环境线索塑造的细胞 Fe-S 簇需求。然而，自近 25 年前首次发现以来，越来越多的证据表明 IscR 的功能远远超出了 Fe-S 簇的动态平衡，不仅在大肠杆菌中，而且在生活方式多样的细菌中也是如此。值得注意的是，病原菌利用 IscR 响应氧张力、氧化和硝化应激以及铁可用性变化的能力，在其各自的宿主中导航其轨迹，因为在宿主感染过程中经常会遇到这些线索的变化。在这篇综述中，我们强调了 IscR 在不同细胞过程中的这些更广泛的作用，并特别讨论了 IscR 作为许多细菌病原体毒力因子的重要性。
