载脂蛋白胆固醇环糊精可提高冷冻斑马鱼（Danio rerio）精子的活力和存活率。
Cholesterol-loaded cyclodextrin improves motility and survival of cryopreserved zebrafish (Danio rerio) sperm.
机构信息
Zebrafish International Resource Center, 5274 University of Oregon, Eugene, OR, 97403, USA.
Zebrafish International Resource Center, 5274 University of Oregon, Eugene, OR, 97403, USA.
出版信息
Cryobiology. 2024 Sep;116:104909. doi: 10.1016/j.cryobiol.2024.104909. Epub 2024 May 25.
We studied the impact of modulating cholesterol levels in zebrafish sperm plasma membranes using cholesterol-loaded methyl-β-cyclodextrin (CLC) and unloaded methyl-β-cyclodextrin (MβC). Zebrafish sperm were treated with these substances before cryopreservation, and post-thaw sperm motility and in vitro fertilization (IVF) rates were compared between treated and untreated samples. Our findings indicate that adding cholesterol to sperm membranes increases post-thaw motility, motile cell count, and motile cell survival within a 0.5-4.0 mg per 1.2 × 10 cell concentration range. Conversely, depleting cholesterol using MβC at 1.0 and 2.0 mg per 1.2 × 10 cells reduced these parameters. On average, all CLC-treated sperm samples produced a 15 % higher IVF rate compared to untreated sperm. Including CLC in the extender before cryopreservation is beneficial for post-thaw sperm quantity and quality in zebrafish.
我们研究了使用载有胆固醇的甲基-β-环糊精（CLC）和未载有胆固醇的甲基-β-环糊精（MβC）来调节斑马鱼精子质膜中胆固醇水平对其的影响。在冷冻保存之前，用这些物质处理斑马鱼精子，然后比较处理和未处理样品的解冻后精子活力和体外受精（IVF）率。我们的研究结果表明，向精子膜中添加胆固醇可增加解冻后精子的活力、活动细胞计数和活动细胞存活率，在 0.5-4.0mg 每 1.2×10 个细胞浓度范围内。相反，使用 1.0 和 2.0mg 每 1.2×10 个细胞的 MβC 耗尽胆固醇会降低这些参数。平均而言，与未处理的精子相比，所有 CLC 处理的精子样本的 IVF 率都提高了 15%。在冷冻保存前将 CLC 包含在延伸剂中有利于斑马鱼解冻后精子的数量和质量。
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