College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
National Center of Technology Innovation for Pigs, Chongqing, 402460, China.
BMC Vet Res. 2024 May 20;20(1):212. doi: 10.1186/s12917-024-04015-w.
Acinetobacter lwoffii (A.lwoffii) is a serious zoonotic pathogen that has been identified as a cause of infections such as meningitis, bacteremia and pneumonia. In recent years, the infection rate and detection rate of A.lwoffii is increasing, especially in the breeding industry. Due to the presence of biofilms, it is difficult to eradicate and has become a potential super drug-resistant bacteria. Therefore, eradication of preformed biofilm is an alternative therapeutic action to control A.lwoffii infection. The present study aimed to clarify that baicalin could eradicate A.lwoffii biofilm in dairy cows, and to explore the mechanism of baicalin eradicating A.lwoffii.
The results showed that compared to the control group, the 4 MIC of baicalin significantly eradicated the preformed biofilm, and the effect was stable at this concentration, the number of viable bacteria in the biofilm was decreased by 0.67 LogCFU/mL. The total fluorescence intensity of biofilm bacteria decreased significantly, with a reduction rate of 67.0%. There were 833 differentially expressed genes (367 up-regulated and 466 down-regulated), whose functions mainly focused on oxidative phosphorylation, biofilm regulation system and trehalose synthesis. Molecular docking analysis predicted 11 groups of target proteins that were well combined with baicalin, and the content of trehalose decreased significantly after the biofilm of A.lwoffii was treated with baicalin.
The present study evaluated the antibiofilm potential of baicalin against A.lwoffii. Baicalin revealed strong antibiofilm potential against A.lwoffii. Baicalin induced biofilm eradication may be related to oxidative phosphorylation and TCSs. Moreover, the decrease of trehalose content may be related to biofilm eradication.
阿克曼氏菌（A.lwoffii）是一种严重的人畜共患病原体，已被确定为脑膜炎、菌血症和肺炎等感染的病因。近年来，A.lwoffii 的感染率和检出率不断上升，尤其是在养殖业中。由于生物膜的存在，难以根除，已成为一种潜在的超级耐药菌。因此，根除已形成的生物膜是控制 A.lwoffii 感染的一种替代治疗方法。本研究旨在阐明黄芩苷可以根除奶牛体内的 A.lwoffii 生物膜，并探讨黄芩苷根除 A.lwoffii 的机制。
结果表明，与对照组相比，黄芩苷的 4 MIC 可显著根除已形成的生物膜，且在该浓度下效果稳定，生物膜内活菌数减少了 0.67 LogCFU/mL。生物膜细菌的总荧光强度显著降低，降低率为 67.0%。共鉴定到 833 个差异表达基因（367 个上调，466 个下调），其功能主要集中在氧化磷酸化、生物膜调控系统和海藻糖合成。分子对接分析预测了 11 组与黄芩苷结合良好的靶蛋白，且黄芩苷处理 A.lwoffii 生物膜后海藻糖含量显著降低。
本研究评估了黄芩苷对 A.lwoffii 的抗生物膜潜力。黄芩苷对 A.lwoffii 表现出很强的抗生物膜潜力。黄芩苷诱导的生物膜根除可能与氧化磷酸化和 TCSs 有关。此外，海藻糖含量的降低可能与生物膜根除有关。
