Hangzhou Key Lab of Inflammation and Immunoregulation, School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Hangzhou Key Lab of Inflammation and Immunoregulation, School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Biochem Biophys Res Commun. 2022 Sep 17;621:130-136. doi: 10.1016/j.bbrc.2022.06.084. Epub 2022 Jul 6.
Natural flavonoids, such as baicalin, have been extensively studied for their role in bacterial infection. However, the underlying mechanisms remain poorly understood. We demonstrated that baicalin coordinates mitochondrial function and dynamics to promote antibacterial response. Baicalin protected against Staphylococcus aureus infections and alleviates inflammatory responses in vivo and in vitro. An increase in mitochondrial mass and elevated expression of factors regulating mitochondrial fission and fusion were observed in baicalin-treated macrophages. Baicalin induced Drp1-dependent biogenesis, which contributes to the generation of additional mitochondria. Baicalin improved the mitochondrial membrane potential, ATP levels, and mitochondrial reactive oxygen species (mtROS) production. Importantly, the inhibition of mitochondrial function by rotenone or MitoTEMPO suppressed the antimicrobial activity of baicalin in macrophages. We conclude that baicalin can regulate immune responses during S. aureus infection by improving mitochondrial function and dynamics, implying that it is a promising therapeutic agent for controlling infection and inflammatory diseases.
天然类黄酮，如黄芩苷，因其在细菌感染中的作用而被广泛研究。然而，其潜在的机制仍知之甚少。我们证明黄芩苷通过协调线粒体功能和动态来促进抗菌反应。黄芩苷可预防金黄色葡萄球菌感染，并减轻体内和体外的炎症反应。在黄芩苷处理的巨噬细胞中观察到线粒体质量增加和调节线粒体分裂和融合的因子表达升高。黄芩苷诱导 Drp1 依赖性生物发生，有助于产生额外的线粒体。黄芩苷提高了线粒体膜电位、ATP 水平和线粒体活性氧（mtROS）的产生。重要的是，用鱼藤酮或 MitoTEMPO 抑制线粒体功能会抑制黄芩苷在巨噬细胞中的抗菌活性。我们得出结论，黄芩苷可以通过改善线粒体功能和动态来调节金黄色葡萄球菌感染期间的免疫反应，这表明它是一种有前途的治疗剂，可以控制感染和炎症性疾病。
