Alkharsah Khaled R
Department of Microbiology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Saudi J Med Med Sci. 2024 Apr-Jun;12(2):182-187. doi: 10.4103/sjmms.sjmms_376_23. Epub 2024 Apr 5.
Viral infection is responsible for the majority of respiratory tract infections (RTI). This retrospective study evaluates the advantages of using the multiplex Xpert Xpress CoV-2/Flu/RSV plus kit in laboratory diagnosis of RTI caused by the most related viruses.
Data were obtained from medical records between November 2021 and May 2023 for any sample tested using either the Xpert Xpress Flu/RSV kit, Xpert Xpress Flu kit, Xpert Xpress SARS-CoV-2 kit, and Xpert Xpress CoV-2/Flu/RSV plus kit.
Influenza virus A was detected in 8.5% (55/649) of the samples using the Flu PCR kit and in 4.9% (123/2538) using CoV-2/Flu/RSV plus kit, while influenza virus B detection rates were 3.7% (24/649) using the Flu PCR kit and 1.7% (43/2538) using the CoV-2/Flu/RSV plus kit. However, the detection rates using the two kits were comparable when evaluated for the same time period of the year. SARS-CoV-2 infections were detected in 16.9% (1545/9153) and 10.5% (266/2538) of the cases using the SARS-CoV-2 kit and CoV-2/Flu/RSV plus kits, respectively. Respiratory syncytial virus (RSV) was identified in 17.7% (66/372) of children using Flu/RSV kit; this number dropped significantly when age-targeted testing of RSV was performed due to involvements of adults. With the CoV-2/Flu/RSV plus kit, about 34% (35/103) of RSV infections detected were in patients aged >20 years; these cases would have previously been overlooked because adults are not routinely tested for RSV using the Flu/RSV kit. All coinfection cases ( = 16) were only detected with the CoV-2/Flu/RSV plus kit.
The use of Xpert Xpress CoV-2/Flu/RSV plus kit not only results in shorter turnaround times through accurate detection of all four viruses, but also provides information on RSV infection in adults and coinfection rates.
病毒感染是大多数呼吸道感染（RTI）的病因。这项回顾性研究评估了使用Xpert Xpress CoV-2/流感/呼吸道合胞病毒（RSV）三联检试剂盒在实验室诊断由最相关病毒引起的呼吸道感染中的优势。
数据取自2021年11月至2023年5月期间的医疗记录，涉及使用Xpert Xpress流感/呼吸道合胞病毒试剂盒、Xpert Xpress流感试剂盒、Xpert Xpress新型冠状病毒2019（SARS-CoV-2）试剂盒以及Xpert Xpress CoV-2/流感/呼吸道合胞病毒三联检试剂盒检测的任何样本。
使用流感PCR试剂盒时，8.5%（55/649）的样本检测到甲型流感病毒，使用CoV-2/流感/呼吸道合胞病毒三联检试剂盒时为4.9%（123/2538）；使用流感PCR试剂盒时，乙型流感病毒的检测率为3.7%（24/649），使用CoV-2/流感/呼吸道合胞病毒三联检试剂盒时为1.7%（43/2538）。然而，在评估一年中的同一时间段时，两种试剂盒的检测率相当。使用SARS-CoV-2试剂盒和CoV-2/流感/呼吸道合胞病毒三联检试剂盒分别在16.9%（1545/9153）和10.5%（266/2538）的病例中检测到SARS-CoV-2感染。使用流感/呼吸道合胞病毒试剂盒在17.7%（66/372）的儿童中检测到呼吸道合胞病毒（RSV）；由于纳入了成人，在进行针对年龄的RSV检测时，这一数字显著下降。使用CoV-2/流感/呼吸道合胞病毒三联检试剂盒，检测到的RSV感染病例中约34%（35/103）为年龄>20岁的患者；这些病例以前会被忽视，因为使用流感/呼吸道合胞病毒试剂盒时通常不会对成人进行RSV检测。所有合并感染病例（n = 16）仅使用CoV-2/流感/呼吸道合胞病毒三联检试剂盒检测到。
使用Xpert Xpress CoV-2/流感/呼吸道合胞病毒三联检试剂盒不仅能通过准确检测所有四种病毒缩短周转时间，还能提供成人RSV感染信息和合并感染率。
