Kim Geon Ho, Chin Koo Bok
Department of Animal Science, Chonnam National University, Gwangju 61186, Korea.
Food Sci Anim Resour. 2024 May;44(3):586-606. doi: 10.5851/kosfa.2024.e2. Epub 2024 May 1.
The study was performed to determine the effect of faba bean protein isolate (FBPI) alone or in combination with microbial transglutaminase (MTG) on the rheological properties of pork myofibrillar protein gel (MPG), and physiochemical and textural properties of reduced-salt, low-fat pork model sausages (LFMSs). The cooking yields of MPGs with MTG or FBPI alone decreased and increased, respectively. However, the combination of FBPI and MTG was similar to the control (CTL) without FBPI or MTG. Gel strength values of MPG added with both FBPI and MTG were higher than treatments with FBPI or MTG alone. The hydrophobicity values of CTL were lower than those of MPG with FBPI alone, whereas the addition of MTG decreased the hydrophobicity of MPGs. The incorporation of FBPI alone or in combination with MTG decreased sulfhydryl groups (p<0.05). Shear stress values of MPGs with MTG tended to be higher than those of non-MTG treatments at all shear rates, and the addition of FBPI into MPGs increased shear stress values. Reduced-salt (1.0%) LFMSs with FBPI alone or combined with MTG had both lower cooking loss and expressible moisture values than those of CTL and similar values to the reference sample (REF, 1.5% salt). Textural properties of reduced-salt LFMSs with FBPI or MTG were similar to those of REF. These results demonstrated that the combination of FBPI and MTG could improve the water binding capacity and textural properties of pork MPGs and LFMSs and might be suitable for application in the development of healthier meat products.
本研究旨在确定单独使用蚕豆分离蛋白(FBPI)或与微生物转谷氨酰胺酶(MTG)联合使用对猪肉肌原纤维蛋白凝胶(MPG)的流变学特性以及低盐低脂猪肉模型香肠(LFMSs)的理化和质地特性的影响。单独使用MTG或FBPI时,MPG的蒸煮产率分别降低和增加。然而,FBPI和MTG的组合与不含FBPI或MTG的对照组(CTL)相似。同时添加FBPI和MTG的MPG的凝胶强度值高于单独使用FBPI或MTG的处理组。CTL的疏水性值低于单独使用FBPI的MPG,而添加MTG降低了MPG的疏水性。单独添加FBPI或与MTG联合添加均降低了巯基含量(p<0.05)。在所有剪切速率下,添加MTG的MPG的剪切应力值往往高于未添加MTG的处理组,并且向MPG中添加FBPI会增加剪切应力值。单独使用FBPI或与MTG联合使用的低盐(1.0%)LFMSs的蒸煮损失和可表达水分值均低于CTL,且与参考样品(REF,1.5%盐)的值相似。添加FBPI或MTG的低盐LFMSs的质地特性与REF相似。这些结果表明,FBPI和MTG的组合可以提高猪肉MPG和LFMSs的水结合能力和质地特性,可能适用于更健康肉类产品的开发。