Combe B, Pope R, Darnell B, Sany J, Talal N
Rev Rhum Mal Osteoartic. 1985 Jun;52(6):385-90.
The activity of natural killer cells in the synovial fluid, the synovial tissue and the peripheral blood was studied in 23 patients with active rheumatoid arthritis and was found to be significantly lower than that in the blood of 28 controls. This decrease was inversely related to the erythrocyte sedimentation rate. The preincubation of mononuclear cells with indomethacin significantly increased the natural killer activity in the blood of the controls and the patients with rheumatoid arthritis, but did not have any effect in the synovial compartment. The elimination of the adherent cells increased the natural killer activity in the blood of the controls and the patients with rheumatoid arthritis, but decreased this activity in the synovium. The stimulatory effect of synovial macrophages and the suppressor effect of the blood macrophages on the natural killer activity were confirmed when the adherent and non-adherent populations were mixed and these effects were reproduced by using supernatants of total mononuclear cells. The stimulation of the natural killer activity by interleukin 2 and poly-I:C, an interferon inducer, is independent of the macrophages in rheumatoid arthritis. These results suggest a deficient natural killer activity in active rheumatoid arthritis and a difference in the modulation of these natural killer cells by macrophages in rheumatoid synovium and normal or rheumatoid arthritis blood.
对23例活动期类风湿关节炎患者的滑液、滑膜组织及外周血中的自然杀伤细胞活性进行了研究，发现其活性显著低于28例对照者血液中的活性。这种降低与红细胞沉降率呈负相关。用吲哚美辛对单核细胞进行预孵育可显著提高对照者及类风湿关节炎患者血液中的自然杀伤细胞活性，但对滑膜腔无任何影响。去除贴壁细胞可提高对照者及类风湿关节炎患者血液中的自然杀伤细胞活性，但会降低滑膜中的该活性。当将贴壁细胞群和非贴壁细胞群混合时，证实了滑膜巨噬细胞对自然杀伤细胞活性的刺激作用及血液巨噬细胞的抑制作用，并且使用总单核细胞的上清液也重现了这些作用。在类风湿关节炎中，白细胞介素2和干扰素诱导剂聚肌胞苷酸对自然杀伤细胞活性的刺激作用不依赖于巨噬细胞。这些结果提示活动期类风湿关节炎患者存在自然杀伤细胞活性缺陷，且类风湿滑膜组织与正常或类风湿关节炎患者血液中的巨噬细胞对这些自然杀伤细胞的调节存在差异。
