Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Postboks 1109 Blindern, 0317 Oslo, Norway.
Clinical Oral Research Laboratory, Institute of Clinical Dentistry, University of Oslo, Norway.
Biomater Sci. 2024 Jun 25;12(13):3345-3359. doi: 10.1039/d4bm00184b.
Nanocoatings based on plant polyphenols have been recently suggested as a potent strategy for modification of implant surfaces for enhancing host cell attachment and reducing bacterial colonisation. In this study we aimed to investigate how serum proteins impact the early adhesion dynamics of human gingival fibroblasts onto titanium surfaces coated with tannic acid (TA). Silicate-TA nanocoatings were formed on titanium and pre-conditioned in medium supplemented with 0, 0.1, 1 or 10% FBS for 1 hour. Dynamics of fibroblasts adhesion was studied using quartz crystal microbalance with dissipation (QCM-D). Time-lapse imaging was employed to assess cell area and motility, while immunofluorescence microscopy was used to examine cell morphology and focal adhesion formation. Our results showed that in serum-free medium, fibroblasts demonstrated enhanced and faster adhesion to TA coatings compared to uncoated titanium. Increasing the serum concentration reduced cell adhesion to nanocoatings, resulting in nearly complete inhibition at 10% FBS. This inhibition was not observed for uncoated titanium at 10% FBS, although cell adhesion was delayed and progressed slower compared to serum-free conditions. In addition, 1% FBS dramatically reduced cell adhesion on uncoated titanium. We revealed a positive relationship between changes in dissipation and changes in cell spreading area, and a negative relationship between dissipation and cell motility. In conclusion, our study demonstrated that serum decreases fibroblasts interaction with surfaces coated with TA in a concentration dependent manner. This suggests that controlling serum concentration can be used to regulate or potentially prevent fibroblasts adhesion onto TA-coated titanium surfaces.
基于植物多酚的纳米涂层最近被提出作为一种有效的策略，用于修饰植入物表面，以增强宿主细胞附着和减少细菌定植。在这项研究中，我们旨在研究血清蛋白如何影响人牙龈成纤维细胞在涂有鞣酸 (TA) 的钛表面上的早期粘附动力学。硅酸钠-TA 纳米涂层在钛上形成，并在补充有 0、0.1、1 或 10% FBS 的培养基中预培养 1 小时。使用石英晶体微天平（QCM-D）研究成纤维细胞的粘附动力学。延时成像用于评估细胞面积和迁移性，而免疫荧光显微镜用于检查细胞形态和焦点粘附形成。我们的结果表明，在无血清培养基中，与未涂层的钛相比，成纤维细胞对 TA 涂层表现出增强和更快的粘附。增加血清浓度会降低细胞对纳米涂层的粘附，导致在 10% FBS 时几乎完全抑制。在 10% FBS 时，未涂层的钛未观察到这种抑制，尽管与无血清条件相比，细胞粘附延迟且进展较慢。此外，1% FBS 显著降低了未涂层钛上的细胞粘附。我们揭示了耗散变化与细胞扩展面积变化之间的正相关关系，以及耗散与细胞迁移性之间的负相关关系。总之，我们的研究表明，血清以浓度依赖的方式降低了与 TA 涂层表面相互作用的成纤维细胞。这表明控制血清浓度可用于调节或潜在预防成纤维细胞粘附到 TA 涂层钛表面。
